Detergent compatible extracellular lipase from streptomyces cellulosae AU-10: A green alternative for the detergent industry
Yükleniyor...
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wıley
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Enzymes can decrease the environmental and economic load of detergent products by reducing the amount of chemicals used in detergents and by allowing washing at ambient temperatures. In this study, Streptomyces cellulosae AU-10 (GenBank accession number: MG780240) lipase was purified 7.08-fold with 68% yield using an aqueous 2-phase system. The Streptomyces sp. AU-10 lipase showed maximal activity at pH 9.0 and 40 degrees C. Hundred percent activities were measured in the pH range from 9.0 to 11.0 for 1 h. The enzyme was also highly stable at 30-50 degrees C. The values of K-m and V-max were calculated as 0.34mM and 0.83mMmin(-1), respectively. The lipase has high hydrolytic activity for olive oil and sunflower oil. The effect of ethylenediamine tetraacetic acid on the enzyme has shown that the lipase is a metalloenzyme. The activity increased in the presence of Fe2+, Cu2+, and various boron compounds. The enzyme has shown a good stability not only with surfactants but also with oxidizing agents. In addition, activities in the presence of Omo, Ariel, Tursil, Pril, and Fairy were measured as 108.8%, 115.6%, 98.35%, 140.4%, and 107.6%, respectively. Considering its remarkable ability, the S. cellulosae AU-10 lipase can be considered as a potential additive in the detergent industry.
Açıklama
Anahtar Kelimeler
Alkaline Lipase, Streptomyces Cellulosae, Biochemical Characterization, Detergent Industry
Kaynak
Journal of Surfactants and Detergents
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
21
Sayı
4
