Total antioxidant capacity, catalase activity, and lipid peroxidation changes in seminal plasma of sex-reversed female and male rainbow trout (Oncorhynchus mykiss) during spawning season

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dc.contributor.authorİnanan, Burak Evren
dc.contributor.authorÖ?retmen, Fatih
dc.contributor.authorİnanan, Tülden
dc.contributor.authorYılmaz, Fevzi
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:30:12Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:30:12Z
dc.date.issued2016
dc.departmentMeslek Yüksekokulu
dc.description.abstractThe advantages of gender-related characteristics are used in aquaculture practice to improve production. For instance, all-female stocic is preferable than mixed or all-male stock in salmonid culture. The most effective way to obtain all-female populations is the using of sex-reversed (SR) female trouts, genotypically female but phenotypically male, by masculinizing androgen hormones as breeders in artificial insemination. This study was conducted to evaluate changes in the total antioxidant capacity (TAC), protein concentration, catalase (CAT) activity, lipid peroxidation level (LPO; malondialdehyde), and Fourier transform infrared spectra of seminal plasma of SR female and normal (N) male trouts during the spawning season. Seminal plasma TAC values of N male and SR female trouts were determined as 0.015 +/- 0.004 and 0.116 +/- 0.033 mM of Trolox equivalents, respectively, in the middle of the spawning season. Some regions related to aromatic rings in seminal plasma Fourier transform infrared spectra of SR female trouts differed from N male trouts were indicated to the higher TAC values. At the middle of the spawning season, protein concentrations were determined as 569.5 +/- 139.4 mg/dL in SR female trouts and 663 +/- 22.7 mg/dL in N male trouts. LPO levels in seminal plasma of N male trouts varied from 46.33 +/- 12.05 x 10(-3) to 270.02 +/- 70.64 x 10(-3) nmolimg protein, whereas from 13.87 +/- 4.98 x 10(-3) to 48.49 +/- 1731 x 10(-3) nmolimg protein in SR female trouts throughout the spawning. CAT activities of seminal plasma in N male trouts ranged from 038 +/- 0.26 to 0.47 +/- 0.32 Ming protein, whereas those values in SR female trouts varied between 0.21 +/- 0.10 and 0.43 +/- 0.15 kU/mg protein. Moreover, there were the pairwise significant correlations among all variables except between CAT and TAC (P > 0.05). Remarkable correlations were found between LPO-protein (r = -0.922, P < 0.05, n = 190), LPO-TAC (r = 0.859, P < 0.05, n = 98), and TAC protein (r = +0.879, P < 0.05, n = 98). Similar to seminal plasma of N male trouts, TAC values, protein concentrations, and CAT activities in seminal plasma of SR female trouts have shown decline, whereas LPO levels increased toward the end of the spawning seasons. Seminal plasmas of SR female trouts were characterized by higher protein concentrations and TAC values and lower LPO levels than that from N male trouts.
dc.description.sponsorshipMugla Sitki Kocman University Research Fund [BAP 2012/92]; Scientific and Technical Research Council of Turkey (TUBITAK) [1130606]
dc.description.sponsorshipThis study has been financially supported by Mugla Sitki Kocman University Research Fund (Grant number: BAP 2012/92) and The Scientific and Technical Research Council of Turkey (TUBITAK) (Grant number: 1130606). The authors also would like to thank personnel from the fish farm manager and the hatchery manager Ersin Celik for technical assistance during sampling.
dc.identifier.doi10.1016/j.theriogenology.2016.06.014
dc.identifier.endpage1982en_US
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.issue8en_US
dc.identifier.pmid27474236
dc.identifier.scopusqualityQ1
dc.identifier.startpage1975en_US
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2016.06.014
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6250
dc.identifier.volume86en_US
dc.identifier.wosWOS:000384628200015
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherELSEVIER SCIENCE INC
dc.relation.ispartofTHERIOGENOLOGY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectSeasonal Variations
dc.subjectSeminal Plasma
dc.subjectRainbow Trout
dc.subjectTotal Antioxidant Capacity
dc.subjectLipid Peroxidation
dc.subjectCatalase Activity
dc.titleTotal antioxidant capacity, catalase activity, and lipid peroxidation changes in seminal plasma of sex-reversed female and male rainbow trout (Oncorhynchus mykiss) during spawning season
dc.typeArticle

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