Microsphere-embedded cryogel for selective and efficient depletion of immunoglobulin G from human serum
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Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Informa Healthcare
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
In this study a novel composite cryogel column was synthesized for affinity depletion of immunoglobulin G (IgG) from human serum. The microsphere-embedding technique combines the large surface area of microspheres with excellent properties of cryogels. The poly(hydroxyethyl methacrylate-N-methacryloyl-L-histidine methyl ester) microsphere (2 m m size)embedded composite cryogel (MECC) column was synthesized and characterized by various methods. The specific surface area of the composite cryogel was found as 92 m(2)/g by using BET measurement. Maximum HIgG adsorption on MECC column was found as 50.5 mg/g. MECC column can be reused many times with no apparent decrease in HIgG adsorption capacity.
Description
Keywords
Composite Cryogel, IgG Affinity Depletion, Microsphere Embedding, Protein Adsorption
Journal or Series
Artificial Cells, Nanomedicine, and Biotechnology
WoS Q Value
N/A
Scopus Q Value
Q1
Volume
41
Issue
5
