Decreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses

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dc.authorid0000-0001-6572-6636
dc.authorid0000-0002-6141-0690
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorKeskin, Nazan
dc.contributor.authorİli, Pınar
dc.contributor.authorBodu, Mustafa
dc.contributor.authorAkalın, Pınar Peker
dc.contributor.authorÖztürk, Ali Erdem
dc.contributor.authorÖzkan, Hüseyin
dc.contributor.authorTopraggaleh, Tohid Rezaei
dc.contributor.authorSarı, Fikret
dc.contributor.authorBaşpınar, Nuri
dc.contributor.authorDursun, Şükrü
dc.date.accessioned2021-06-16T11:03:46Z
dc.date.available2021-06-16T11:03:46Z
dc.date.issued2020
dc.departmentVeteriner Fakültesi
dc.description*Dursun, Şükrü ( Aksaray, Yazar )
dc.description.abstractThis study aimed to evaluate the comparative effects of taxifolin hydrate and trehalose on the quality of frozen-thawed ram spermatozoa for the first time. Ejaculates collected from six mature rams were pooled, and divided to eight equal aliquots to extend them with different concentrations of glycerol (%5 and %3), taxifolin hydrate (10, 100, and 500 ?M), and trehalose (60 mM) as eight groups (G5T0, G5T10, G5T100, G5T500, G3T0, G3T10, G3T100, and G3T500). After freeze-thawing process of cryopreservation, microscopic and oxidative stress parameters, and gene expression levels were investigated for understanding of possible impacts of taxifolin hydrate and trehalose. The study showed that G3T10 resulted in the highest post-thawed viability and mitochondrial activity. Moreover, all extenders with taxifolin hydrate reduced DNA fragmentation in comparison to G5T0, but DNA damage was prevented at the highest rate in presence of G5T10. The level of LPO significantly decreased in the groups G5T500 and G3T100, and the expression levels of NQO1, GCLC, and GSTP1 genes significantly increased in the groups G5T100, G5T500, G3T10, and G3T100 compared to the group G5T0. Finally, co-supplementation of tris-based extender having 3% glycerol with 60 mM trehalose and 10 ?M taxifolin hydrate in cryopreservation extender may be recommended to improve the quality of post-thawed ram spermatozoa. However, further in vivo and in vitro studies are suggested to evaluate fertility rates of frozen-thawed ram spermatozoa co-supplemented with trehalose and taxifolin hydrate.
dc.identifier.doi10.1016/j.cryobiol.2020.09.001
dc.identifier.endpage29en_US
dc.identifier.issn0011-2240
dc.identifier.issue-en_US
dc.identifier.pmid32890464
dc.identifier.scopusqualityQ1
dc.identifier.startpage19en_US
dc.identifier.urihttps:/dx.doi.org/10.1016/j.cryobiol.2020.09.001
dc.identifier.urihttps://hdl.handle.net/20.500.12451/8121
dc.identifier.volume96en_US
dc.identifier.wosWOS:000579825400003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Inc.
dc.relation.ispartofCryobiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAntioxidants
dc.subjectCryopreservation
dc.subjectGene Expression
dc.subjectOxidative Stress
dc.subjectRam Semen
dc.subjectSperm Parameters
dc.subjectTaxifolin Hydrate
dc.subjectTrehalose
dc.titleDecreasing glycerol content by co-supplementation of trehalose and taxifolin hydrate in ram semen extender: Microscopic, oxidative stress, and gene expression analyses
dc.typeArticle

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