Effects of arginine and trehalose on post-thawed bovine sperm quality

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dc.contributor.authorÖztürk, Caner
dc.contributor.authorGüngör, Şükrü
dc.contributor.authorAtaman, Mehmet Bozkurt
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorBaşpınar, Nuri
dc.contributor.authorİli, Pınar
dc.contributor.authorİnanc, Muhammed Enes
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:17:46Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:17:46Z
dc.date.issued2017
dc.departmentVeteriner Fakültesi
dc.description.abstractThe present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and diluted at 37 degrees C with base extenders containing 2 mM arginine, 25 mM trehalose and no antioxidant (control) was cooled to 5 degrees C and then frozen. Frozen straws were thawed in a water bath for evaluation. Supplementation of the semen extender with arginine decreased the percentages of post-thawed subjective motility (29 +/- 8.21%), CASA motility (12.2 +/- 5.69%) and progressive motility (3.52 +/- 2.13%), compared with the controls (43 +/- 2.73%, 55.4 +/- 6.78% and 33.48 +/- 4.14%, respectively, P < 0.05). Supplementation of the semen extender with trehalose produced a higher mitochondrial activity and sperm viability (36.3 +/- 3.99% and 44.1 +/- 2.18%) compared with the control (13 +/- 8.15 and 31.7 +/- 3.94%, respectively, P < 0.05). It was established that trehalose (95.1%) and arginine (92.8%) protect DNA integrity compared to the control (90.4%) (P < 0.05). Trehalose supplementation in semen extenders provided great benefit in terms of viability, mitochondrial activity, and intact sperm DNA on frozen-thawed bull sperm.
dc.identifier.doi10.1556/004.2017.040
dc.identifier.endpage439en_US
dc.identifier.issn0236-6290
dc.identifier.issn1588-2705
dc.identifier.issue3en_US
dc.identifier.pmid28956482
dc.identifier.scopusqualityQ2
dc.identifier.startpage429en_US
dc.identifier.urihttps://doi.org/10.1556/004.2017.040
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4793
dc.identifier.volume65en_US
dc.identifier.wosWOS:000411907900010
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAkademiai Kiado Rt.
dc.relation.ispartofActa Veterinaria Hungarica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectArginine
dc.subjectBull Sperm
dc.subjectCOMET
dc.subjectFluorescent Staining
dc.subjectTrehalose
dc.subjectOxidative Stress
dc.titleEffects of arginine and trehalose on post-thawed bovine sperm quality
dc.typeArticle

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