Preparation and characterization of magnetically responsive bacterial polyester based nanospheres for cancer therapy

Basit Öğe Kaydı
dc.authoridSAGLAM, NECDET -- 0000-0002-5463-8355; SAM, Mesut -- 0000-0002-6429-7144
dc.contributor.authorErdal, Ebru
dc.contributor.authorKavaz, Doğa
dc.contributor.authorŞam, Mesut
dc.contributor.authorDemirbilek, Murat
dc.contributor.authorDemirbilek, Melike Erol
dc.contributor.authorSağlam, Necdet
dc.contributor.authorDenkbaş, Emir Baki
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:26:21Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:26:21Z
dc.date.issued2012
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractPolyhydroxyalkanoates (PHA) are natural, thermoplastic polyesters and due to their biocompatible and biodegradable properties they are good alternatives for the production of scaffolds for engineered tissues or nanoparticles for drug delivery. As a member of polyhydroxyalkanoate family, polyhydroxybutyrates (PHB) have been widely used as a biomaterial for in vitro and in vivo studies since their mechanical properties are very similar to conventional plastics. By using multi-emulsion technique, iron oxide particles were coated with polyhydroxybutyrate (PHB) polymer synthesized from Alcaligenes eutrophus bacteria and the magnetic carrier system was prepared accordingly. The bare nanoparticles and magnetic nanoparticles were morphologically, structurally and magnetically characterized by using Scanning electron microscope (SEM) and Atomic force microscope (AFM); Fourier Transform Infrared Spectrometry (FTIR), and Electron Spin Resonance (ESR) and Vibrating Sample Magnetometer (VSM) techniques, respectively. Particle size of PHB nanoparticles was determined by Zeta Sizer. It was found that the smallest particles were in the range of 239,43+/-5,25 nm in diameter. Concanavalin-A (Con-A) was used for targeting the Cancer cells while etoposide was used as drug. Con-A and etoposide were loaded onto the particles. Release studies of etoposide were evaluated and the system was optimized for the further in vivo applications. Finally different formulation magnetic PHB nanoparticles cytotoxicity were evaluated in cell culture studies and used He La cell line (cervical cancer cells) as a cancer cells and L929 cells (mouse fibroblast cells) as a non-cancer cell line.
dc.description.sponsorshipHacettepe University Scientific Researches Unit
dc.description.sponsorshipThis project was supported by Hacettepe University Scientific Researches Unit and the authors would like to thank Bilkent University, Institute of Materials Science and Nanotechnology (UNAM).
dc.identifier.doi10.1166/jbn.2012.1431
dc.identifier.endpage808en_US
dc.identifier.issn1550-7033
dc.identifier.issue5en_US
dc.identifier.pmid22888751
dc.identifier.scopusqualityQ2
dc.identifier.startpage800en_US
dc.identifier.urihttps://doi.org/10.1166/jbn.2012.1431
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5551
dc.identifier.volume8en_US
dc.identifier.wosWOS:000306941400011
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAmer Scientific Publishers
dc.relation.ispartofJournal of Biomedical Nanotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectPolyhydroxybutyrate
dc.subjectMagnetically Responsive Nanospheres
dc.subjectCancer Therapy
dc.subjectEtaposide
dc.subjectConcanavalin-A
dc.titlePreparation and characterization of magnetically responsive bacterial polyester based nanospheres for cancer therapy
dc.typeArticle

Dosyalar

Koleksiyon

Makale Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu