Exploring the combined anti-cancer effects of sodium butyrate and celastrol in glioblastoma cell lines: a novel therapeutic approach

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dc.authorid0000-0002-7330-9231
dc.authorid0000-0002-7598-2665
dc.contributor.authorKartal, Bahar
dc.contributor.authorDenizler Ebiri, Farika Nur
dc.contributor.authorGüven, Mustafa
dc.contributor.authorTaşpınar, Filiz
dc.contributor.authorCanpınar, Hande
dc.contributor.authorÇetin, Sedat
dc.contributor.authorKaraduman, Tuğçe
dc.contributor.authorKüçüktürk, Serkan
dc.contributor.authorCastresana, Javier
dc.contributor.authorTaşpınar, Mehmet
dc.date.accessioned2024-07-03T13:05:03Z
dc.date.available2024-07-03T13:05:03Z
dc.date.issued2024
dc.departmentTıp Fakültesi
dc.description.abstractGlioblastoma, a highly aggressive and lethal brain cancer, lacks effective treatment options and has a poor prognosis. In our study, we explored the potential anti-cancer effects of sodium butyrate (SB) and celastrol (CEL) in two glioblastoma cell lines. SB, a histone deacetylase inhibitor, and CEL, derived from the tripterygium wilfordii plant, act as mTOR and proteasome inhibitors. Both can cross the blood–brain barrier, and they exhibit chemo- and radiosensitive properties in various cancer models. GB cell lines LN-405 and T98G were treated with SB and CEL. Cell viability was assessed by MTT assay and IC50 values were obtained. Gene expression of DNA repair, apoptosis, and autophagy-related genes was analyzed by RT-PCR. Cell cycle distribution was determined using flow cytometry. Viability assays using MTT assay revealed IC50 values of 26 mM and 22.7 mM for SB and 6.77 ?M, and 9.11 ?M for CEL in LN-405 and T98G cells, respectively. Furthermore, we examined the expression levels of DNA repair genes (MGMT, MLH-1, MSH-2, MSH-6), apoptosis genes (caspase-3, caspase-8, caspase-9), and an autophagy gene (ATG-6) using real-time polymerase chain reaction. Additionally, flow cytometry analysis revealed alterations in cell cycle distribution following treatment with SB, CEL and their combination. These findings indicate that SB and CEL may act through multiple mechanisms, including DNA repair inhibition, apoptosis induction, and autophagy modulation, to exert their anti-cancer effects in glioblastoma cells. This is the first study providing novel insights into the potential therapeutic effects of SB and CEL in glioblastoma.
dc.identifier.doi10.1007/s12032-024-02340-6
dc.identifier.issn1357-0560
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ2
dc.identifier.urihttps:/dx.doi.org/10.1007/s12032-024-02340-6
dc.identifier.urihttps://hdl.handle.net/20.500.12451/12039
dc.identifier.volume41en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMedical Oncology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectApoptosis
dc.subjectAutophagy
dc.subjectCelastrol
dc.subjectDNA Repair
dc.subjectGlioblastoma
dc.subjectSodium Butyrate
dc.titleExploring the combined anti-cancer effects of sodium butyrate and celastrol in glioblastoma cell lines: a novel therapeutic approach
dc.typeArticle

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