Cu2+-attached pumice particles embedded composite cryogels for protein purification

Basit Öğe Kaydı
dc.authoridAlkan, Huseyin -- 0000-0002-0761-6436
dc.contributor.authorAlkan, Hüseyin
dc.contributor.authorCömert, Şeyda Ceylan
dc.contributor.authorGürbüz, Fatma
dc.contributor.authorDoğru, Mehmet İlker
dc.contributor.authorOdabaşı, Mehmet
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:30:08Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:30:08Z
dc.date.issued2017
dc.departmentMühendislik Fakültesi
dc.description.abstractIn this study, chromatographic performance of Cu2+-attached pumice particles embedded to monolithic cryogels (Cu2+-APPsEMC) for human serum albumin (HSA) was investigated. Monolithic composite cryogels were prepared by means of polymerization of gel-forming precursors at sub-zero temperatures. The chemical composition of pumice and surface of composite cryogels were determined by X-ray fluorescence spectrometer and scanning electron microscopy, respectively. The highest adsorption capacity (549.5 mg/g pumice) of cryogels was achieved at phosphate buffer of pH 8.0 with initial HSA solution of 3 mg/ml. SDS-PAGE analysis was performed for the samples studied on human serum to determine HSA adsorption/desorption performance of cryogel qualitatively.
dc.identifier.doi10.3109/21691401.2015.1129627
dc.identifier.endpage97en_US
dc.identifier.issn2169-1401
dc.identifier.issn2169-141X
dc.identifier.issue1en_US
dc.identifier.pmid26755205
dc.identifier.scopusqualityQ1
dc.identifier.startpage90en_US
dc.identifier.urihttps://doi.org/10.3109/21691401.2015.1129627
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6243
dc.identifier.volume45en_US
dc.identifier.wosWOS:000391459800014
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Group
dc.relation.ispartofArtificial Cells, Blood Substitutes, and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectComposite Cryogel
dc.subjectHuman Serum Albumin Chromatography
dc.subjectMetal Chelate
dc.subjectParticle Embedding
dc.subjectPumice
dc.titleCu2+-attached pumice particles embedded composite cryogels for protein purification
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
alkan-huseyin-2017.pdf
Boyut:
6.67 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu