A novel one?step multiplex PCR protocol to detect avian haemosporidian parasites in the subgenus Haemoproteus (Kruse, 1890) used to quantify parasite prevalence in domestic pigeons (Columba livia) in Turkey

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dc.contributor.authorÇiloğlu, Arif
dc.contributor.authorYıldırım, Alparslan
dc.contributor.authorPekmezci, Didem
dc.contributor.authorYetişmiş, Gamze
dc.contributor.authorSürsal Şimsek, Neslihan
dc.contributor.authorŞimşek, Emrah
dc.contributor.authorDüzlü, Önder
dc.contributor.authorÖnder, Zuhal
dc.contributor.authorDelibaşı Kokçü, Nesrin
dc.contributor.authorPekmezci, Gökmen Zafer
dc.contributor.authorEllis, Vincenzo A.
dc.contributor.authorİnci, Abdullah
dc.date.accessioned2022-06-29T06:07:54Z
dc.date.available2022-06-29T06:07:54Z
dc.date.issued2023
dc.departmentVeteriner Fakültesi
dc.description.abstractInfections of avian haemosporidian parasites are regularly identified by molecular methods including multiplex PCR, which allows researchers to distinguish mixed infections of parasites from multiple genera. Here we extend the utility of a previously designed multiplex PCR by designing a primer set specific to parasites of the subgenus Haemoproteus (genus: Haemoproteus). The updated one-step multiplex PCR protocol we describe here allows for the detection of the genera Plasmodium and Leucocytozoon and the two subgenera (Haemoproteus and Parahaemoproteus) of the genus Haemoproteus. A sensitivity analysis showed that the multiplex PCR could amplify DNA of parasites in the subgenus Haemoproteus at very low levels of infection. We used this multiplex PCR to identify haemosporidian infections in 250 adult domestic pigeons (Columba livia) in Turkey. All samples were also screened by microscopy and a widely used nested PCR to compare with the results of multiplex PCR, to detect low levels of parasitemia, and to identify possible abortive infections. In total, 71 pigeons (28.4%) were found to be infected by all three methods. The multiplex PCR protocol successfully detected and discriminated both subgenera Haemoproteus and Parahaemoproteus infections. We compared our results with previous host species records to assess the host specificity of the parasite lineages we found. Our findings provide novel data on the prevalence of avian haemosporidians in domestic pigeons and demonstrate the utility of the new one-step multiplex PCR protocol for the determination of mixed avian haemosporidian infections. We expect that this protocol will contribute to a better understanding of the distribution, epizootiology, and ecology of avian haemosporidians.
dc.identifier.doi10.1007/s11259-022-09962-z
dc.identifier.endpage-en_US
dc.identifier.issue-en_US
dc.identifier.pmid35739341
dc.identifier.scopusqualityQ2
dc.identifier.startpage-en_US
dc.identifier.urihttps:/dx.doi.org/10.1007/s11259-022-09962-z
dc.identifier.urihttps://hdl.handle.net/20.500.12451/9513
dc.identifier.volume-en_US
dc.identifier.wosWOS:000814974200001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofVeterinary Research Communications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectHaemoproteus
dc.subjectLeucocytozoon
dc.subjectParahaemoproteus
dc.subjectParasite Detection
dc.subjectPigeons
dc.subjectPlasmodium
dc.titleA novel one?step multiplex PCR protocol to detect avian haemosporidian parasites in the subgenus Haemoproteus (Kruse, 1890) used to quantify parasite prevalence in domestic pigeons (Columba livia) in Turkey
dc.typeArticle

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