IMAC application of extracellular polymeric substances doped composite membranes for ?-amylase immobilization and kinetic studies
| dc.contributor.author | Bani, Nurullah | |
| dc.contributor.author | İnanan, Tülden | |
| dc.contributor.author | Acet, Ömür | |
| dc.contributor.author | Odabaşı, Mehmet | |
| dc.date.accessioned | 2024-07-02T12:23:39Z | |
| dc.date.available | 2024-07-02T12:23:39Z | |
| dc.date.issued | 2024 | |
| dc.department | Sabire Yazıcı Fen Edebiyat Fakültesi | |
| dc.description.abstract | Immobilized metal affinity chromatography (IMAC), extensively utilized technique in affinity chromatography, has proven to be highly effective in purifying ?-amylase, a crucial enzyme in industrial applications. Here, Poly (2-hydroxyethyl methacrylate) (PHEMA) based composite membranes were fabricated using the radical copolymerization technique in a petri dish. Extracellular polymeric materials (EPS) as the metal binding agent. The attachment of Cu2+ ions to the membranes occurred through the utilization of EPSs (Cu2+-EPS). The membranes having Cu2+-EPS (Cu2+-EPS-PM) were subjected to various characterization techniques, including scanning electron microscopy (SEM), attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR), swelling tests, and surface area measurements. The investigation also encompassed the examination of various factors, including pH, initial ?-amylase concentration, temperature, and ionic strength, that exert an influence on the adsorption of ?-amylase from an aqueous medium. The Cu2+-EPS-PMs demonstrated maximum adsorption capacity with a value of 54.2 mg/g polymer (pH 5.0 sodium acetate buffer, temperature: 25°C, initial ?-amylase concentration of 2 mg/mL). Additionally, it has been observed that the enzyme is able to be used in adsorption and desorption cycles on Cu2+-EPS-PM many times. The investigation also focused on examining the impact of pH, temperature, storage, and operational stability over the activities of both free and immobilized ?-amylase. The outcomes showed that immobilization had positive effects on activity. | |
| dc.identifier.doi | 10.1016/j.mcat.2024.114198 | |
| dc.identifier.issn | 2468-8231 | |
| dc.identifier.scopusquality | Q1 | |
| dc.identifier.uri | https:/dx.doi.org/10.1016/j.mcat.2024.114198 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12451/12020 | |
| dc.identifier.volume | 562 | en_US |
| dc.identifier.wosquality | N/A | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.language.iso | en | |
| dc.publisher | Elsevier B.V. | |
| dc.relation.ispartof | Molecular Catalysis | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/embargoedAccess | |
| dc.subject | Extracellular Polymeric Materials (EPS) | |
| dc.subject | Immobilized Metal Affinity Chromatography | |
| dc.subject | Kinetic Activity | |
| dc.subject | Membrane | |
| dc.subject | ?-amylase | |
| dc.title | IMAC application of extracellular polymeric substances doped composite membranes for ?-amylase immobilization and kinetic studies | |
| dc.type | Article |
