DNA damage, oxidative stress, decreased viability and motility in common carp (Cyprinuscarpio l.) spermatozoa induced by tryptophan, phenylalanine and cysteine amino acids during short-term storage
Yükleniyor...
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Türkiye Klinikleri
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Free amino acids are found in spermatozoa and seminal plasma. Their qualitative and quantitative presence have been affected by many factors. Under in vitro storage conditions, their effects on spermatozoa in animal species and also human have been concerned over the last decades. In this study, the effects of tryptophan, phenylalanine and cysteine on DNA integrity, lipid peroxidation, viability and motility parameters in spermatozoa of the common carp (Cyprinuscarpio) were assessed during short-term storage. Spermatozoa were incubated at 1, 5, 25, and 50 mM of these amino acids in vitro at 4 °C in a time?dependent manner (6, 24, and 48 h). The supplementation of cysteine, phenylalanine and >5 mM of tryptophan to the incubation medium attenuated the spermatozoa motility parameters and viability. Particularly, >1 mM of cysteine completely inhibited progressive motility and decreased viability to <50%. Cysteine and phenylalanine at all concentrations significantly caused the increases in lipid peroxidation products in spermatozoa and seminal plasma, and hereby induced the percentage of DNA damage in spermatozoa. Cysteine had more detrimental effects than phenylalanine and tryptophan. Our data provide evidence that these amino acids are not beneficial for the maintaining of carp spermatozoa at 4 °C.
Free amino acids are found in spermatozoa and seminal plasma. Their qualitative and quantitative presence have been affected by many factors. Under in vitro storage conditions, their effects on spermatozoa in animal species and also human have been concerned over the last decades. In this study, the effects of tryptophan, phenylalanine and cysteine on DNA integrity, lipid peroxidation, viability and motility parameters in spermatozoa of the common carp (Cyprinuscarpio) were assessed during short-term storage. Spermatozoa were incubated at 1, 5, 25, and 50 mM of these amino acids in vitro at 4 °C in a time?dependent manner (6, 24, and 48 h). The supplementation of cysteine, phenylalanine and >5 mM of tryptophan to the incubation medium attenuated the spermatozoa motility parameters and viability. Particularly, >1 mM of cysteine completely inhibited progressive motility and decreased viability to <50%. Cysteine and phenylalanine at all concentrations significantly caused the increases in lipid peroxidation products in spermatozoa and seminal plasma, and hereby induced the percentage of DNA damage in spermatozoa. Cysteine had more detrimental effects than phenylalanine and tryptophan. Our data provide evidence that these amino acids are not beneficial for the maintaining of carp spermatozoa at 4 °C.
Free amino acids are found in spermatozoa and seminal plasma. Their qualitative and quantitative presence have been affected by many factors. Under in vitro storage conditions, their effects on spermatozoa in animal species and also human have been concerned over the last decades. In this study, the effects of tryptophan, phenylalanine and cysteine on DNA integrity, lipid peroxidation, viability and motility parameters in spermatozoa of the common carp (Cyprinuscarpio) were assessed during short-term storage. Spermatozoa were incubated at 1, 5, 25, and 50 mM of these amino acids in vitro at 4 °C in a time?dependent manner (6, 24, and 48 h). The supplementation of cysteine, phenylalanine and >5 mM of tryptophan to the incubation medium attenuated the spermatozoa motility parameters and viability. Particularly, >1 mM of cysteine completely inhibited progressive motility and decreased viability to <50%. Cysteine and phenylalanine at all concentrations significantly caused the increases in lipid peroxidation products in spermatozoa and seminal plasma, and hereby induced the percentage of DNA damage in spermatozoa. Cysteine had more detrimental effects than phenylalanine and tryptophan. Our data provide evidence that these amino acids are not beneficial for the maintaining of carp spermatozoa at 4 °C.
Açıklama
Anahtar Kelimeler
Cystein, Cystein, eDNA damage, eDNA damage, Oxidative Stress, Oxidative Stress, Phenylalanine, Phenylalanine, Spermatozoa Motility, Spermatozoa Motility, Tryptophan, Tryptophan
Kaynak
Turkish Journal of Zoology
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
44
Sayı
3
