?-Amylase immobilized composite cryogels: Some studies on kinetic and adsorption factors
| dc.authorid | 0000-0003-2408-8660 | |
| dc.contributor.author | Acet, Ömür | |
| dc.contributor.author | İnanan, Tülden | |
| dc.contributor.author | Önal Acet, Burcu | |
| dc.contributor.author | Dikici, Emrah | |
| dc.contributor.author | Odabaşı, Mehmet | |
| dc.date.accessioned | 2021-06-24T20:15:39Z | |
| dc.date.available | 2021-06-24T20:15:39Z | |
| dc.date.issued | 2021 | |
| dc.department | Teknik Bilimler Meslek Yüksekokulu | |
| dc.description | *İnanan, Tülden (Aksaray, Yazar ) *Önal Acet, Burcu (Aksaray, Yazar ) * Dikici, Emrah (Aksaray, Yazar ) *Odabaşı, Mehmet (Aksaray, Yazar ) | |
| dc.description.abstract | Stability of enzymes is a significant factor for their industrial feasibility. ?-Amylase is an important enzyme for some industries, i.e., textile, food, paper, and pharmaceutics. Pumice particles (PPa) are non-toxic, natural, and low-cost alternative adsorbents with high adsorption capacity. In this study, Cu2+ ions were attached to pumice particles (Cu2+-APPa). Then, Cu2+-APPa embedded composite cryogel was synthesized (Cu2+-APPaC) via polymerization of gel-forming agents at minus temperatures. Characterization studies of the Cu2+-APPaC cryogel column were performed by X-ray fluorescence spectrometry (XRF), scanning electron microscopy (SEM), and Brunauer, Emmett, Teller (BET) method. The experiments were carried out in a continuous column system. ?-Amylase was adsorbed onto Cu2+-APPaC cryogel with maximum amount of 858.7 mg/g particles at pH 4.0. Effects of pH and temperature on the activity profiles of the free and the immobilized ?-amylase were investigated, and results indicate that immobilization did not alter the optimum pH and temperature values. kcat value of the immobilized ?-amylase is higher than that of the free ?-amylase while KM value increases by immobilization. | |
| dc.identifier.doi | 10.1007/s12010-021-03559-z | |
| dc.identifier.endpage | - | en_US |
| dc.identifier.issn | 0273-2289 | |
| dc.identifier.issue | - | en_US |
| dc.identifier.scopusquality | Q2 | |
| dc.identifier.startpage | - | en_US |
| dc.identifier.uri | https:/dx.doi.org/10.1007/s12010-021-03559-z | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12451/8180 | |
| dc.identifier.volume | - | en_US |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | PubMed | |
| dc.language.iso | en | |
| dc.publisher | Springer | |
| dc.relation.ispartof | Applied Biochemistry and Biotechnology | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | Bead Embedding | |
| dc.subject | Composite Cryogel | |
| dc.subject | IMAC | |
| dc.subject | Protein Adsorption | |
| dc.subject | ?-Amylase | |
| dc.title | ?-Amylase immobilized composite cryogels: Some studies on kinetic and adsorption factors | |
| dc.type | Article |
