Gold nanoparticles loaded TNF-? and CALNN peptide as a drug delivery system and promising therapeutic agent for breast cancer cells
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor and Francis Ltd.
Erişim Hakkı
info:eu-repo/semantics/embargoedAccess
Özet
We investigated the anti-cancer properties of gold nanoparticles loaded TNF- and CALNN peptides, which we proposed as a potential drug delivery system using in vitro and in vivo models. The binding of GNPs-TNF- and GNPs-TNF-CALNN was characterized using a UV, ELISA and SEM analysis. The outcomes demonstrated that a novel drug delivery system had an anti-proliferative activity against breast cancer cell lines through a mechanism of apoptosis induction. In vivo model involved studying the cytotoxic influence of a drug delivery system GNPs, GNPs-TNF-? and GNPs-TNF-?-CALNN when applied to the transplanted AN-3 cell line. tumor sections were examined using microarray. In-vivo studies demonstrated that GNPs alone had less of a growth inhibitory effect on tumors implanted in mice when compared to GNPs-TNF–CALNN combined therapy. The cytotoxic assay showed that GNPs, GNPs-TNF-? and GNPs-TNF-?-CALNN exhibit selective toxicity towards cancer cells, inducing cell apoptosis through activation of caspase-3 and 7, p53 protein.
Açıklama
Anahtar Kelimeler
Apoptosis, Breast Cancer, CALNN, Gold Nanoparticles, TNF- ?
Kaynak
Materials Technology
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
37
Sayı
14
