Influence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing ability

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dc.authoridTUNCER, Purhan Barbaros -- 0000-0002-9257-9544
dc.contributor.authorSarıözkan, Serpil
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorTuncer, Pürhan B.
dc.contributor.authorBüyükleblebici, Serhat
dc.contributor.authorCantürk, Fazile
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:27:03Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:27:03Z
dc.date.issued2014
dc.departmentVeterinerlik Fakültesi
dc.description.abstractSupplementation of the semen extender with antioxidants did not produce any significant effect on CASA and progressive motilities and sperm motility characteristics, in comparison to the control group (P > 0.05). For sperm acrosome and total abnormalities, TCM-199 supplemented with cysteine (2.60 +/- 0.24% and 4.80 +/- 0.20%), glutamine (2.80 +/- 0.20% and 6.40 +/- 0.40%), carnitine (2.60 +/- 0.24% and 6.00 +/- 0.63%) and methionine (3.40 +/- 0.51% and 9.20 +/- 0.86%) at doses of 2 mM provided a better protective effect, compared to that of the controls (8.00 +/- 0.44 and 15.60 +/- 1.895). As regards sperm membrane integrity, supplementation with 2 mM of glutamine and methionine (56.00 +/- 1.70% and 62.40 +/- 1.78%, respectively) resulted in higher rates, when compared to the control group (41.40 +/- 4.74%). According to the results of the COMET assay, only the use of TCM-199 supplemented with 2 mM of cysteine reduced DNA damage and resulted in percentages of sperm with damaged DNA (2.17 +/- 0.18%) lower than those of the control group (3.16 +/- 0.32%) (P < 0.001). For pregnancy rates, there were no significant differences among the extender groups (P > 0.05). Crown Copyright (C) 2014 Published by Elsevier Inc. All rights reserved
dc.identifier.doi10.1016/j.cryobiol.2014.01.007
dc.identifier.endpage133en_US
dc.identifier.issn0011-2240
dc.identifier.issn1090-2392
dc.identifier.issue1en_US
dc.identifier.pmid24468272
dc.identifier.scopusqualityQ1
dc.identifier.startpage129en_US
dc.identifier.urihttps://doi.org/10.1016/j.cryobiol.2014.01.007
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5778
dc.identifier.volume68en_US
dc.identifier.wosWOS:000332190100018
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofCryobiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectBovine
dc.subjectAntioxidants
dc.subjectSperm Parameters
dc.subjectDNA Damage
dc.subjectFreeze-thawing
dc.titleInfluence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing ability
dc.typeArticle

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