Detailed adsorption mechanism of plasmid DNA by newly isolated cellulose from waste flower spikes of Thypa latifolia using quantum chemical calculations

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dc.authoridSargin, Idris -- 0000-0003-3785-9575; Mujtaba, Muhammad -- 0000-0001-8392-9226; Kaya, Murat -- 0000-0001-6954-2703; AKYUZ YILMAZ, Bahar -- 0000-0001-9760-9856
dc.contributor.authorMujtaba, Muhammad
dc.contributor.authorKaya, Murat
dc.contributor.authorAkyüz, Lalehan
dc.contributor.authorErdönmez, Demet
dc.contributor.authorAkyüz, Bahar
dc.contributor.authorSargın, İdris
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:17:46Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:17:46Z
dc.date.issued2017
dc.department[Mujtaba, Muhammad -- Kaya, Murat -- Akyuz, Bahar -- Sargin, Idris] Aksaray Univ, Fac Sci & Letters, Dept Biotechnol & Mol Biol, TR-68100 Aksaray, Turkey -- [Erdonmez, Demet] Aksaray Univ, Fac Sci & Letters, Dept Biol, TR-68100 Aksaray, Turkey -- [Akyuz, Lalehan] Aksaray Univ, Tech Vocat Sch, Dept Chem Technol, TR-68100 Aksaray, Turkey
dc.description.abstractCurrent study was designed to use the newly obtained cellulose from waste flower spikes of Thypa latifolia plant for plasmid DNA adsorption. Cellulose was isolated according to a previously described method including acid and base treatment, and cellulose content was recorded as 17%. T. latifolia cellulose was physicochemically characterized via FT-IR, TGA and SEM techniques. Detailed mechanism of plasmid DNA adsorption by newly isolated cellulose was described using chemical quantum calculations. To check the effect of Cu++ immobilization on the affinity of cellulose for plasmid DNA, copper ions were immobilized onto T. latifolia cellulose. pUC18 plasmid DNA was used for adsorption studies. Membranes prepared with only T. latifolia cellulose and Cu++ immobilized T. latifolia cellulose revealed different adsorption ratios as 43.9 and 86.9% respectively. This newly isolated cellulose from waste flower spikes of T. latifolia can be utilized as a suitable carrier for plasmid DNA. (C) 2017 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.ijbiomac.2017.04.104
dc.identifier.endpage923en_US
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid28457957
dc.identifier.scopusqualityQ1
dc.identifier.startpage914en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2017.04.104
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4792
dc.identifier.volume102en_US
dc.identifier.wosWOS:000406984300100
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevıer Scıence Bv
dc.relation.ispartofInternatıonal Journal of Bıologıcal Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectpUC18
dc.subjectSEM
dc.subjectCu++ Immobilization
dc.subjectTGA
dc.subjectFT-IR
dc.titleDetailed adsorption mechanism of plasmid DNA by newly isolated cellulose from waste flower spikes of Thypa latifolia using quantum chemical calculations
dc.typeArticle

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