Immunohistochemical and immunocytochemical findings associated with Marek's disease virus in naturally infected laying hens

Basit Öğe Kaydı
dc.contributor.authorYavuz, Orhan
dc.contributor.authorErer, H.
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:18:11Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:18:11Z
dc.date.issued2017
dc.departmentVeterinerlik Fakültesi
dc.description.abstractWe compared immunohistochemical (IHC) staining of tissue sections of liver, kidney, spleen, lung, proventriculus, sciatic nerve, bursa of Fabricius, brain, heart, intestine and skin; immunocytochemical (ICC) staining of peripheral blood samples and touch preparations of liver, spleen and kidney of laying hens naturally infected with Marek's disease (MD) virus. We used one hundred and fifty 5-17-week-old commercial hens. IHC and ICC staining were performed using polymer-based techniques. IHC staining exhibited mostly free immunopositive reactions in tumor cells and in the cytoplasm of the parenchymal cells of liver, kidney, spleen and bursa of Fabricius. In the sciatic nerve, severe reactions were observed in the cytoplasm of plasma and MD cells in the lymphoproliferative areas. Pronounced staining was found in the lymphoid cells in the medulla of intrafollicular regions in the bursa of Fabricius. Although immunostaining was observed in the liver and spleen touch preparations, there was no staining in the kidneys and peripheral blood cell samples. The presence of virus in the tissue and peripheral blood samples and in touch preparations was compared immunohistochemically and immunocytochemically. IHC and ICC techniques were helpful for diagnosis of MD. Peripheral blood samples are inappropriate for field conditions and natural infections.
dc.description.sponsorshipSelcuk University Scientific Research Projects Committee [10102022]
dc.description.sponsorshipThis study funded by Selcuk University Scientific Research Projects Committee, project number 10102022.
dc.identifier.doi10.1080/10520295.2017.1359750
dc.identifier.endpage505en_US
dc.identifier.issn1052-0295
dc.identifier.issn1473-7760
dc.identifier.issue7en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage498en_US
dc.identifier.urihttps://doi.org/10.1080/10520295.2017.1359750
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4847
dc.identifier.volume92en_US
dc.identifier.wosWOS:000424145900005
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis
dc.relation.ispartofBiotechnic and Histochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHens
dc.subjectImmunocytochemistry
dc.subjectImmunohistochemistry
dc.subjectMarek's Disease
dc.subjectTumor
dc.titleImmunohistochemical and immunocytochemical findings associated with Marek's disease virus in naturally infected laying hens
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
yavuz-orhan-2017.pdf
Boyut:
1.04 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu