Assessment of toxicity and oxidative DNA damage of sodium hypochlorite, chitosan and propolis on fibroblast cells

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dc.contributor.authorUğur Aydın, Zeliha
dc.contributor.authorAkpınar, Kerem Engin
dc.contributor.authorHepokur, Ceylan
dc.contributor.authorErdönmez, Demet
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:17:36Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:17:36Z
dc.date.issued2018
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractThe objective of this study was to evaluate and compare the cytotoxicity and genotoxicity on human fibroblast cell lines of sodium hypochlorite (NaOCl), chitosan and propolis as root canal irrigating solutions. Human fibroblast cells were exposed to chitosan, propolis and NaOCl for 4 and 24 h. Cell viability was assessed by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide, and oxidative DNA damage was assessed by determination of 8-hydroxydeoxyguanosine (8-OHdG) level with an ELISA kit. The data of cell cytotoxicity were analysed statistically using a test of one-way analysis of variance at a significance level of p < 0.05. In the NaOCI group, the 8-OHdG level was higher than in the chitosan group, but there was no statistical difference when compared with the other groups (p < 0.05). It was determined that the irrigation solutions were cytotoxic, depending on the dose and time. NaOCl was the most toxic solution after both 4and 24 h of exposure (p < 0.05). Chitosan and propolis may be alternatives to NaOCl for irrigation solutions, because they are both less toxic and produce less oxidative DNA damage.
dc.description.sponsorshipScientific Research Project Fund of Cumhuriyet University [Dis-167]
dc.description.sponsorshipThis work is supported by the Scientific Research Project Fund of Cumhuriyet University under the project number Dis-167.
dc.identifier.doi10.1590/1807-3107bor-2018.vol32.0119
dc.identifier.issn1807-3107
dc.identifier.pmid30517428
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1590/1807-3107bor-2018.vol32.0119
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4772
dc.identifier.volume32en_US
dc.identifier.wosWOS:000467760500020
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSociedade Brasileira De Pesquisa Odontologica
dc.relation.ispartofBrazilian Oral Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChitosan
dc.subjectPropolis
dc.subjectSodium Hypochlorite
dc.titleAssessment of toxicity and oxidative DNA damage of sodium hypochlorite, chitosan and propolis on fibroblast cells
dc.typeArticle

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