In vitro evaluation of antisense oligonucleotide functionalized core-shell nanoparticles loaded with -tocopherol succinate

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dc.contributor.authorKılıçay, Ebru
dc.contributor.authorKarahalıloğlu, Zeynep
dc.contributor.authorAlpaslan, Pınar
dc.contributor.authorHazer, Baki
dc.contributor.authorDenkbaş, Emir Baki
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:18:03Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:18:03Z
dc.date.issued2017
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractAntisense oligonucleotide (ASO)-conjugated--tocopherol succinate (TCS)-loaded-poly(lactic acid)-g-poly(ethylene glycol) nanoparticles (ASO-TCS-PLA-PEG NPs), with the ratio of polymer/TCS of 10:2.5, 10:5, 10:7 (w/w) were prepared for targeting cancer therapy. The amphiphilic PLA, amino terminated PEG graft copolymers were synthesized by ring opening polymerization reaction. Nanoparticles were produced by using double emulsion (w/o/w) solvent evaporation method. ASO-TCS-PLA-PEG NPs demonstrated satisfactory encapsulation and loading efficiency and size distribution. The short-term stability studies were carried out at 4 and 25 degrees C for 30days to assess their mean particle size, polydispersity index and zeta potential. The cellular uptake and extended cytoplasmic retention of the NPs in A549 human lung carcinoma and L929 mouse fibroblast cells were examined by fluorescence and confocal microscopy. In human lung cancer cells, ASO-TCS-PLA-PEG NPs exhibited better cellular internalization, cytotoxicity and apoptotic and necrotic effects compared to healthy cell line, L929. These findings showed that ASO-modified nanoparticles could serve as a promising nanocarrier for targeted tumor cells.
dc.description.sponsorshipBulent Ecevit University [BEU-2017-YKD-33496813-01]
dc.description.sponsorshipThis work was financially supported by Bulent Ecevit University [BEU-2017-YKD-33496813-01].
dc.identifier.doi10.1080/09205063.2017.1354670
dc.identifier.endpage1785en_US
dc.identifier.issn0920-5063
dc.identifier.issn1568-5624
dc.identifier.issue15en_US
dc.identifier.pmid28696185
dc.identifier.scopusqualityQ1
dc.identifier.startpage1762en_US
dc.identifier.urihttps://doi.org/10.1080/09205063.2017.1354670
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4829
dc.identifier.volume28en_US
dc.identifier.wosWOS:000407503900010
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis
dc.relation.ispartofJournal Of Biomaterials Science-Polymer Edition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectPLA-PEG
dc.subjectAntisense Oligonucleotide
dc.subjectTocopherol Succinate
dc.subjectDrug Delivery
dc.subjectHuman Lung Cancer Cells
dc.titleIn vitro evaluation of antisense oligonucleotide functionalized core-shell nanoparticles loaded with -tocopherol succinate
dc.typeArticle

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