Investigation of the Effects of Silymarin on Ovarian Ischemia Reperfusion via Nrf-2/HO-1/NQO1, Ki-67 and Wnt Signaling Pathways
| dc.authorid | 0000-0001-7773-5978 | |
| dc.authorid | 0000-0002-8457-9448 | |
| dc.contributor.author | Öztürk, Ayşe Betül | |
| dc.contributor.author | Akaras, Nurhan | |
| dc.contributor.author | Şimşek, Hasan | |
| dc.contributor.author | Kandemir, Fatih Mehmet | |
| dc.date.accessioned | 2025-07-17T10:42:38Z | |
| dc.date.available | 2025-07-17T10:42:38Z | |
| dc.date.issued | 2025 | |
| dc.department | Tıp Fakültesi | |
| dc.description.abstract | Ovarian ischemia is a pathological condition that usually occurs due to ovarian torsion, resulting in the interruption of blood supply to the ovaries and oxygen deficiency. Silymarin (SLM) is a flavonoid complex of plant origin with pharmacological properties such as antioxidant, anti-inflammatory, and antiapoptotic effects. In this study, we investigated the effects of SLM through different pathways in rats subjected to experimental ovarian ischemia/reperfusion (I/R). Female Wistar rats were divided into five groups: Control, SLM (50 mg/kg), I/R, I/R + SLM25 (25 mg/kg), and I/R + SLM50 (50 mg/kg). SLM was given orally for 7 days, followed by ischemia (2 h) and reperfusion (2 h) on day 8. Biochemical (MDA, GSH, SOD, CAT, GPx) and histological (H&E, Ki-67 IHC) analyses were performed. Also, molecular (qRT-PCR) analyses were performed to evaluate oxidative stress, inflammation, apoptosis, and Wnt signaling. I/R increased MDA and NO levels in ovarian tissue while decreasing SOD, CAT, GPx, and GSH. Antioxidant defense genes (Nrf-2, HO-1, NQO1) were suppressed, and inflammation markers (NF-ĸB, IL-1β, TNF-α) along with apoptotic markers (Bax, Caspase-3) were elevated, while Bcl-2 decreased. The Wnt signaling pathway was inhibited, particularly at Wnt-3A, LRP5, Dvl-2, and Cyclin-1, reducing Ki-67 protein levels and IHC positivity. Silymarin has shown a therapeutic effect on ovarian ischemia reperfusion injury with its antioxidant, antiapoptotic and anti-inflammatory effects and cell cycle regulatory activity. | |
| dc.identifier.doi | 10.1002/jbt.70138 | |
| dc.identifier.issn | 10956670 | |
| dc.identifier.issue | 1 | |
| dc.identifier.pmid | 39812109 | |
| dc.identifier.scopus | 85215314966 | |
| dc.identifier.uri | https://dx.doi.org/10.1002/jbt.70138 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12451/13352 | |
| dc.identifier.volume | 39 | |
| dc.identifier.wos | WOS:001395899400001 | |
| dc.identifier.wosquality | Q3 | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | Web of Science | |
| dc.institutionauthor | Öztürk, Ayşe Betül | |
| dc.institutionauthor | Akaras, Nurhan | |
| dc.institutionauthor | Şimşek, Hasan | |
| dc.institutionauthor | Kandemir, Fatih Mehmet | |
| dc.institutionauthorid | 0000-0001-7773-5978 | |
| dc.institutionauthorid | 0000-0002-8457-9448 | |
| dc.language.iso | en | |
| dc.publisher | John Wiley and Sons Inc | |
| dc.relation.ispartof | Journal of Biochemical and Molecular Toxicology | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | Apoptosis | |
| dc.subject | Inflammation | |
| dc.subject | Ischemia/reperfusion | |
| dc.subject | Ovarium | |
| dc.subject | Silymarin | |
| dc.subject | Wnt | |
| dc.title | Investigation of the Effects of Silymarin on Ovarian Ischemia Reperfusion via Nrf-2/HO-1/NQO1, Ki-67 and Wnt Signaling Pathways | |
| dc.type | Article |
