The Effects of Storage Conditions and Homogenisation Buffers on the Measurement of SOD, CAT and ADA Enzyme Activities in Cattle Liver

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dc.authorid0000-0001-8813-4991
dc.contributor.authorBalkan, Burcu Menekşe
dc.contributor.authorMeral, Öğünç
dc.contributor.authorÇetintav, Bekir
dc.contributor.authorTutun, Hidayet
dc.contributor.authorÖzkurt, Güzin
dc.date.accessioned2025-07-09T07:07:17Z
dc.date.available2025-07-09T07:07:17Z
dc.date.issued2025
dc.departmentVeteriner Fakültesi
dc.description.abstractBackground: Accurate measurement of enzyme activities is very important in studies to evaluate enzymatic parameters. While performing these measurements, many factors can affect the results, including the method of obtaining the tissues, the conditions under which they are stored until analysis, and the methods of determining enzyme activity. Objectives: This study aimed to investigate the effect of different storage conditions (time and temperature) and different homogenisation buffers (PBS or KCl) on the enzymatic activity of superoxide dismutase (SOD), catalase (CAT), and adenosine deaminase (ADA) in homogenised cattle liver. Methods: Fresh liver tissue samples were obtained from the slaughterhouse and homogenised in different homogenisation buffers. Supernatants from each sample were divided into three groups according to the experimental design of the study. SOD, CAT and ADA enzyme activities in homogenised tissues were evaluated. Results: Our data revealed that SOD, CAT and ADA activities did not differ significantly between PBS and KCl groups or between -20°C and -80°C freezing temperatures. However, our results showed that SOD levels decreased over time in both groups, CAT levels demonstrated a significant decrease from Month 0 to Month 3 and ADA levels decreased significantly over time. Conclusions: The results indicated that storage time had a significant effect on enzyme activity changes, but the effect of storage temperature and homogenisation buffer was generally limited. These results may support the measurement of enzymatic activity in liver homogenate immediately and, if necessary, after freezing for the shortest possible time.
dc.identifier.doi10.1002/vms3.70418
dc.identifier.issn20531095
dc.identifier.issue4
dc.identifier.pmid40434928
dc.identifier.scopus105006732976
dc.identifier.urihttps://dx.doi.org/10.1002/vms3.70418
dc.identifier.urihttps://hdl.handle.net/20.500.12451/13212
dc.identifier.volume11
dc.identifier.wosWOS:001497664500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakPubMed
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.institutionauthorÖzkurt, Güzin
dc.language.isoen
dc.publisherJohn Wiley and Sons Inc
dc.relation.ispartofVeterinary Medicine and Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAdenosine Deaminase
dc.subjectCatalase
dc.subjectCattle
dc.subjectEnzymatic Activity
dc.subjectLiver
dc.subjectSuperoxide Dismutase
dc.titleThe Effects of Storage Conditions and Homogenisation Buffers on the Measurement of SOD, CAT and ADA Enzyme Activities in Cattle Liver
dc.typeArticle

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