Autologous conditioned serum increases fat graft viability mmore than platelet-rich plasma in a controlled rat model
| dc.contributor.author | Baykara, Gökay | |
| dc.contributor.author | Sungur, Nezih | |
| dc.contributor.author | Özer, Kadri | |
| dc.contributor.author | Atan, Oğuz | |
| dc.contributor.author | Caydere, Muzaffer | |
| dc.contributor.author | Koşar, Pınar Nercis | |
| dc.contributor.author | Koçer, Uğur | |
| dc.date.accessioned | 2022-08-25T06:32:09Z | |
| dc.date.available | 2022-08-25T06:32:09Z | |
| dc.date.issued | 2022 | |
| dc.department | Tıp Fakültesi | |
| dc.description.abstract | Platelet-rich plasma has been used to support fat graft retention, but it may include inflammatory mediators such as interleukin-1?. Autologous conditioned serum also contains high levels of various anti-inflammatory cytokines. The authors hypothesized that combining autologous conditioned serum with fat graft would increase fat graft survival more than platelet-rich plasma. Methods: Twenty-seven adult, male, Sprague-Dawley rats were divided into three groups of nine. Ten nonstudy rats were used to prepare platelet-rich plasma, autologous conditioned serum, and fat grafts. Next, 0.7-ml fat graft with a combination of 0.2 ml of autologous conditioned serum, platelet-rich plasma, or phosphate-buffered saline was applied to their dorsa. Fat graft volume was assessed on postoperative day 2 and on the day of euthanization at 1, 3, and 5 months postoperatively. Histopathologic analysis was performed to measure integrity, inflammation, fibrosis, and vascularization. Results: The median volume percentages and interquartile ranges at 1 month postoperatively were 97.3 percent (77.3 to 119.6 percent), 40.4 percent (30.9 to 46.9 percent), and 72.1 percent (53.6 to 84.9 percent) in autologous conditioned serum plus fat graft, phosphate-buffered saline plus fat graft, and platelet-rich plasma plus fat graft, respectively (p < 0.05); at 3 months postoperatively, values were 82.3 percent (70.3 to 88.3 percent), 36.6 percent (29.4 to 43.1 percent), and 48.3 percent (31.4 to 57.9 percent) (p < 0.001); and at 5 months postoperatively, values had increased to 83.9 percent (58.3 to 102.4 percent), 40.3 percent (20.1 to 50.6 percent), and 56.3 percent (37.7 to 74.9 percent), respectively (p < 0.05). Conclusions: Autologous conditioned serum and platelet-rich plasma improved fat graft outcomes compared to saline, whereas autologous conditioned serum was associated with less inflammation, greater fat viability, and more integrity. Clinical Relevance Statement: Combining fat graft with autologous conditioned serum may be a better option to minimize resorption rate and improve graft survival. | |
| dc.identifier.doi | 10.1097/PRS.0000000000009029 | |
| dc.identifier.endpage | 1136 | en_US |
| dc.identifier.issn | 0032-1052 | |
| dc.identifier.issue | 5 | en_US |
| dc.identifier.pmid | 35271553 | |
| dc.identifier.scopusquality | Q1 | |
| dc.identifier.startpage | 1123 | en_US |
| dc.identifier.uri | https:/dx.doi.org/10.1097/PRS.0000000000009029 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12451/9644 | |
| dc.identifier.volume | 149 | en_US |
| dc.identifier.wos | WOS:000788119300043 | |
| dc.identifier.wosquality | Q1 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | PubMed | |
| dc.language.iso | en | |
| dc.publisher | Lippincott Williams and Wilkins | |
| dc.relation.ispartof | Plastic and Reconstructive Surgery | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/embargoedAccess | |
| dc.subject | Autologous Conditioned Serum Increases | |
| dc.subject | Fat Graft Viability | |
| dc.title | Autologous conditioned serum increases fat graft viability mmore than platelet-rich plasma in a controlled rat model | |
| dc.type | Article |
