Effect of cholesterol loaded cyclodextrin on semen cryopreservation of Aksaray Malakli shepherd dogs of different ages

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dc.authoridDurmaz, Emre -- 0000-0003-2239-1762
dc.contributor.authorİnanç, Muhammed Enes
dc.contributor.authorTekin, Koray
dc.contributor.authorOlgaç, Kemal Tuna
dc.contributor.authorYılmaz, Burak
dc.contributor.authorÇil, Beste
dc.contributor.authorTaşdemir, Umut
dc.contributor.authorTuncer, Purhan Barbaros
dc.contributor.authorBüyükleblebici Serhat
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:17:04Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:17:04Z
dc.date.issued2018
dc.departmentAksaray Teknik Bilimler Meslek Yüksekokulu
dc.description.abstractThe objective of the study was to determine the effect of cholesterol-loaded cyclodextrin (CLC) on the quality parameters of semen from Aksaray Malakli Shepherd dogs of different age groups. Forty-eight male dogs were divided into 3 groupings according to their ages (young age (Y): <= 3 years, n: 20; middle age (M): 4-6 years, n: 20; old age (O): >= 7 years; n: 8). The sperm-rich portion of the ejaculate from each dog was divided into four aliquots and extended with either tris as a control (C) or tris loaded with 0.5, 1.0, and 1.5 mg/120 x 10(6) CLC as low (L), intermediate (I), and high (H) doses, respectively. Following equilibration for at least half an hour, the straws were frozen in nitrogen vapor and then stored in liquid nitrogen at least for 48 h. Later, the frozen straws were thawed in a water bath for spermatological evaluation. Significant differences were observed between different age groups in terms of the spermatological parameters (p < 0.05). The evidence suggests that increasing age is associated with poor in-vitro spermatological parameters and CLC was able to protect the acrosome integrity from cryo-damage during the freeze-thawing process. Better semen freezability characteristics were obtained at young ages, considering the overall parameters.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [1140636]
dc.description.sponsorshipThis study was financed under the Project supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No:1140636).
dc.identifier.doi10.1016/j.anireprosci.2018.04.068
dc.identifier.endpage200en_US
dc.identifier.issn0378-4320
dc.identifier.issn1873-2232
dc.identifier.pmid29699920
dc.identifier.scopusqualityQ1
dc.identifier.startpage191en_US
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2018.04.068
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4697
dc.identifier.volume193en_US
dc.identifier.wosWOS:000433999000021
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevıer Scıence Bv
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAge
dc.subjectCholesterol
dc.subjectCyclodextrin
dc.subjectMalakli Shepherd Dog
dc.subjectSperm Freezing
dc.titleEffect of cholesterol loaded cyclodextrin on semen cryopreservation of Aksaray Malakli shepherd dogs of different ages
dc.typeArticle

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