The effects of different doses of ROCK inhibitor, antifreeze protein III, and boron added to semen extender on semen freezeability of Ankara bucks

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dc.authorid0000-0002-7598-2665
dc.contributor.authorKaraşör, Ömer Faruk
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorCenariu, Mihai
dc.contributor.authorBodu, Mustafa
dc.contributor.authorTaşpınar, Mehmet
dc.contributor.authorTaşpınar, Filiz
dc.date.accessioned2023-01-11T06:20:54Z
dc.date.available2023-01-11T06:20:54Z
dc.date.issued2022
dc.departmentTeknik Bilimler Meslek Yüksekokulu
dc.description.abstractIn the presented study, the effects of ROCK inhibitor Y-27632, antifreeze protein III, and boron at two different doses were investigated on the spermatological parameters of Ankara buck semen after freeze–thawing. Ejaculates were collected from bucks using an electroejaculator during the breeding season. The ejaculates that showed appropriate characteristics were pooled and used in the dilution and freezing of semen. The extender groups were formed by adding two different doses of three different additives (ROCK inhibitor Y-27632, 5 and 20 µM; antifreeze protein III, 1 and 4 µg/mL; boron, 0.25 and 1 mM) to the control extender. The semen was diluted with the different extenders at 35–37 °C and loaded into straws. Sperm samples frozen in liquid nitrogen vapors, following equilibration, were stored in liquid nitrogen. It was observed that extender supplementation improved post-thaw motility of Ankara buck semen after freeze–thawing. Differences were significant (p < 0.01) for 5 and 10 µM doses of ROCK inhibitor (71.82% and 74.04 % motility), as well as for 0.25 and 1 mM doses of boron (76.36% and 72.08% motility), compared to the control group (66.15% motility). With respect to the evaluation of acrosomal integrity and mitochondrial activity after freeze–thawing, although supplementation provided protection at all doses, the efficacy was not statistically significant (p > 0.05). It was observed that DNA damage was improved by antifreeze protein III at 1 µg/mL (1.23% ± 0.23%) and by boron at all doses (0.25 mM: 1.83% and 1 mM: 1.18%) compared to the control group (3.37%) (p < 0.01), following the thawing process. In the present study, it was determined that some additives added to the extender provided significant improvements in buck spermatozoa motility and DNA damage after thawing.
dc.identifier.doi10.3390/molecules27228070
dc.identifier.endpage-en_US
dc.identifier.issn1420-3049
dc.identifier.issue22en_US
dc.identifier.pmid36432171
dc.identifier.scopusqualityQ1
dc.identifier.startpage-en_US
dc.identifier.urihttps:/dx.doi.org/10.3390/molecules27228070
dc.identifier.urihttps://hdl.handle.net/20.500.12451/9865
dc.identifier.volume27en_US
dc.identifier.wosWOS:000887403400001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMDPI
dc.relation.ispartofMolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnkara Buck
dc.subjectAntifreeze Protein III
dc.subjectBoron
dc.subjectROCK Inhibitor
dc.subjectSemen Freezing
dc.titleThe effects of different doses of ROCK inhibitor, antifreeze protein III, and boron added to semen extender on semen freezeability of Ankara bucks
dc.typeArticle

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