The effect of green tea extract supplementation in bull semen cryopreservation

Basit Öğe Kaydı
dc.contributor.authorİnanç, Muhammed Enes
dc.contributor.authorÇil, Beste
dc.contributor.authorYeni, Deniz
dc.contributor.authorAvdatek, Fatih
dc.contributor.authorOrakçı, Durmuş
dc.contributor.authorTuncer, Purhan Barbaros
dc.contributor.authorTürkmen, Ruhi
dc.contributor.authorTaşdemir, Umut
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:16:08Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:16:08Z
dc.date.issued2019
dc.departmentAksaray Teknik Bilimler Meslek Yüksekokulu
dc.description.abstractThis study aims to investigate the effect of catechin (CT), green tea extract, as a supplement to Tris extender on semen quality parameters in frozen-thawed of bull sperm. Ejaculates were taken with artificial vagina from Holstein bulls and divided equal five aliquots, diluted to containing different amounts of CT (5, 10, 25 and 50 mu g/mL) and no-additive (control). All samples were equilibrated at 4 degrees C for 4 h and were frozen using a digital freezing machine. Post-thawed sperm motility and kinetic parameters were determined using the sperm analyser system. Spermatozoa DNA integrity was evaluated with the single cell gel electrophoresis, abnormal spermatozoa rate was evaluated by fluid fixation test and lipid peroxidation status was evaluated calorimetrically. CT supplementation did not improve motility and kinetic parameters. However, the higher morphological integrity was detected in CT10, 25 and 50 groups compared to control (P<0.05). Regarding chromatin integrity, positive effects of catechin were observed in the treatment groups while in CT 50 group adverse effects were found (P<0.05). Although there was no improvement in malondialdehyde levels, the highest total antioxidant activity was seen in the CT50 group (P<0.05). In conclusion, CT supplementation could be used the protection of morphological and DNA integrity from cryodamage and it has increased the total antioxidant activity depending of the dose in bull semen.
dc.description.sponsorshipGeneral Directorate of Agribusiness, Ankara, Turkey
dc.description.sponsorshipThis study was supported by General Directorate of Agribusiness, Ankara, Turkey.
dc.identifier.doi10.9775/kvfd.2019.21702
dc.identifier.endpage708en_US
dc.identifier.issn1300-6045
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ3
dc.identifier.startpage703en_US
dc.identifier.urihttps://doi.org/10.9775/kvfd.2019.21702
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4541
dc.identifier.volume25en_US
dc.identifier.wosWOS:000471814000017
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherKafkas Üniversitesi Veteriner Fakültesi Dergisi
dc.relation.ispartofKafkas Üniversitesi Veteriner Fakültesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBull Semen Freezing
dc.subjectCatechin
dc.subjectDNA Integrity
dc.subjectLipid Peroxidation
dc.titleThe effect of green tea extract supplementation in bull semen cryopreservation
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
inanc-muhammed enes-2019.pdf
Boyut:
236.54 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu