Pumice particle interface: a case study for immunoglobulin G purification
| dc.contributor.author | Alacabey, ,İhsan | |
| dc.contributor.author | Acet, Ömür | |
| dc.contributor.author | Önal, Burcu | |
| dc.contributor.author | Dikici, Emrah | |
| dc.contributor.author | Karakoç, Veyis | |
| dc.contributor.author | Gürbüz, Fatma | |
| dc.contributor.author | Alkan, Hüseyin | |
| dc.contributor.author | Odabaşı, Mehmet | |
| dc.date.accessioned | 2021-06-24T10:43:03Z | |
| dc.date.available | 2021-06-24T10:43:03Z | |
| dc.date.issued | 2020 | |
| dc.department | Teknik Bilimler Meslek Yüksekokulu | |
| dc.description.abstract | Cryogels with embedded natural adsorbent are new trend of chromatographic media for separation of biomolecules. In this report, experimental determination of immunoglobulin G (IgG) purification by Cu2+-attached pumice particles unified cryogel (Cu2+-PPUC) was performed. For this purpose, after preparation of Cu2+-attached pumice particles, they were unified with 2-hydroxyethyl methacrylate monomers to produce Cu2+-PPUC through polymerization of gel-forming precursors at subzero temperatures. IgG separation experiments were accomplished in a continuous column system. The highest binding capacity (596.8 mg/g) was obtained by working with 0.02 M phosphate buffer at pH 6.0. The chemical analysis of pumice was examined by X-ray fluorescence spectrometer. Scanning electron microscopy was performed to identify the morphology of Cu2+-PPUC. Langmuir adsorption model was best fitted to interaction when compared to Freundlich model. Temkin model was utilized to characterize adsorption, energetically. Purification ability of Cu2+-PPUC for IgG was shown with high selectivity via reducing SDS–PAGE electrophoresis. | |
| dc.identifier.doi | 10.1007/s00289-020-03392-0 | |
| dc.identifier.endpage | - | en_US |
| dc.identifier.issn | 0170-0839 | |
| dc.identifier.issue | - | en_US |
| dc.identifier.scopusquality | Q1 | |
| dc.identifier.startpage | - | en_US |
| dc.identifier.uri | https:/dx.doi.org/10.1007/s00289-020-03392-0 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12451/8169 | |
| dc.identifier.volume | - | en_US |
| dc.identifier.wos | WOS:000575725000003 | |
| dc.identifier.wosquality | Q2 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.language.iso | en | |
| dc.publisher | Springer Science and Business Media Deutschland GmbH | |
| dc.relation.ispartof | Polymer Bulletin | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/embargoedAccess | |
| dc.subject | Composite Cryogels | |
| dc.subject | IgG Separation | |
| dc.subject | IMAC | |
| dc.subject | Pumice Particles | |
| dc.title | Pumice particle interface: a case study for immunoglobulin G purification | |
| dc.type | Article |
