Critical-size alveolar defect treatment via TGF-ss 3 and BMP-2 releasing hybrid constructs

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dc.authoridİnci, İlyas -- 0000-0001-7231-7822
dc.contributor.authorAlıcı Garipcan, Aybüke
dc.contributor.authorKorkusuz, Petek
dc.contributor.authorBilgiç, Elif
dc.contributor.authorAşkın, Kerem
dc.contributor.authorAydın, Halil M.
dc.contributor.authorÖztürk, Eda
dc.contributor.authorİnci, İlyas
dc.contributor.authorÖzkızılcık, Asya
dc.contributor.authorÖztürk, Kamile
dc.contributor.authorPişkin, Erhan
dc.contributor.authorVargel, İbrahim
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:16:25Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:16:25Z
dc.date.issued2019
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractIn the present study a combination of Transforming Growth Factor Beta 3 (TGF-beta 3) and Bone Morphogenetic Protein-2 (BMP-2) loaded gelatin films sandwiched between poly (L-lactide) (PLLA)/poly (epsilon-caprolactone) (PCL) matrices were produced to enhance bone formation in alveolar bone defects. Osteogenic properties of tissue constructs were tested in alveolar bone defect model in rats. Bone healing was assessed by osteogenic gene expression levels of bone sialoprotein (BSP), alkaline phosphatase (ALP), osteonectin (ON, SPARC), osteocalcin (OC), runt-related transcription factor 2 (RUNX2), bone specific alkaline phosphatase (BALP) activity, histomorphometry and microtomography. Increase in osteogenic gene expression levels and BALP activity results showed that new bone formation was significantly accelerated in TGF-beta 3 + BMP-2 loaded scaffold group compared to growth factor free and only BMP-2 loaded groups. The micro-computed tomography (mu-CT) data from the 4(th) months revealed that (TGF-beta 3+ BMP-2) loaded scaffolds displayed increased bone formation and was able to fulfill 84% of the defect area (p < 0.05). Accelerated bone formation in the S-GF-B-T group compared to that of the S-GF group at the end of the 4th month was further verified via histomorphometric analysis (p = 0.008). Gene expression, BALP activity, microtomography and histomorphometry analysis indicated that (TGF-beta 3 + BMP-2) loaded PLLA/PCL scaffolds increased the new bone formation. BMP-2 loaded scaffolds were less effective than combination of TGF-beta 3 and BMP-2 loaded scaffolds. These findings demonstrated that focusing on the PLLA/PCL hybrid scaffolds combined with (TGF-beta 3 + BMP-2) may lay the groundwork for future therapy-oriented efforts to enhance bone formation in alveolar defects.
dc.description.sponsorshipTurkish Scientific and Technological Research Council (TUBITAK) [108S336, SBAG-4083]
dc.description.sponsorshipWe thank Professor Hamdi H. Alkan, MD to provide using the mu-CT facility. This study was funded by the Turkish Scientific and Technological Research Council (TUBITAK) Project No: 108S336 (SBAG-4083). Erhan Piskin is a full member of Turkish Academy of Sciences.
dc.identifier.doi10.1080/09205063.2019.1571397
dc.identifier.endpage436en_US
dc.identifier.issn0920-5063
dc.identifier.issn1568-5624
dc.identifier.issue5en_US
dc.identifier.pmid30688157
dc.identifier.scopusqualityQ1
dc.identifier.startpage415en_US
dc.identifier.urihttps://doi.org/10.1080/09205063.2019.1571397
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4591
dc.identifier.volume30en_US
dc.identifier.wosWOS:000462355600005
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofJournal of Biomaterials Science-Polymer Edition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAlveolar Bone Defects
dc.subjectTGF-Beta 3
dc.subjectBMP-2
dc.subjectPLLA
dc.subjectPCL
dc.subjectGelatin
dc.titleCritical-size alveolar defect treatment via TGF-ss 3 and BMP-2 releasing hybrid constructs
dc.typeArticle

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