Cryopreservation of ram semen: baicalein efficiency on oxidative stress, chromatin integrity, viability and motility post thaw
| dc.contributor.author | Avdatek, Fatih | |
| dc.contributor.author | Güngör, Şükrü | |
| dc.contributor.author | Gülhan, Mehmet Fuat | |
| dc.contributor.author | İnanç, Muhammed Enes | |
| dc.contributor.author | Olğaç, Kemal Tuna | |
| dc.contributor.author | Denk, Barış | |
| dc.contributor.author | Yeni, Deniz | |
| dc.contributor.author | Taşdemir, Umut | |
| dc.date.accessioned | 2024-07-19T11:06:46Z | |
| dc.date.available | 2024-07-19T11:06:46Z | |
| dc.date.issued | 2024 | |
| dc.department | Teknik Bilimler Meslek Yüksekokulu | |
| dc.description.abstract | Baicalein (B) has potential antioxidant properties, but it has not been tested as a ram semen extender. This study aimed to assess the impact of B on various sperm parameters and determine its potential influence on semen quality after the freeze-thawing process. During the breeding season, ejaculates were obtained from four rams with the aid of an artificial vagina. The collected mixed semen samples were divided into four groups: control (C; 0), B0.5 (0.5 mM), B1 (1 mM), and B2 (2 mM). After semen extension, the samples were loaded into 0.25 mL straws and stored for 2 h at 4°C prior to freezing in liquid nitrogen vapor and thawed in a water bath at 37°C. Among the groups, B0.5 demonstrated the highest progressive motility results, while B1 and B2 exhibited reduced motility (p < 0.05). In terms of high mitochondrial membrane potential, plasma membrane and acrosome integrity, and viability, B0.5 showed significantly superior outcomes to the other B groups (p < 0.05), although it was not significantly better than C. B1 displayed the highest plasma membrane integrity levels (p < 0.05). Notably, B2 displayed the lowest total antioxidant status levels among the groups (p < 0.05). The findings of this study suggested that the in vitro spermatological characteristics of ram spermatozoa such as progressive motility and chromatin integrity can be protected from the freeze-thawing process by using the 0.5 mM dose of baicalein as a semen extender. The treatment of sperm freezing might benefit from further in-depth research on the role of B in the improvement of cryoinjury and its underlying processes. | |
| dc.identifier.doi | 10.3389/fvets.2024.1394273 | |
| dc.identifier.issn | 2297-1769 | |
| dc.identifier.issue | - | en_US |
| dc.identifier.scopusquality | Q1 | |
| dc.identifier.uri | https:/dx.doi.org/10.3389/fvets.2024.1394273 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12451/12173 | |
| dc.identifier.volume | 11 | en_US |
| dc.identifier.wosquality | N/A | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | PubMed | |
| dc.language.iso | en | |
| dc.publisher | Frontiers Media SA | |
| dc.relation.ispartof | Frontiers in Veterinary Science | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | Antioxidant | |
| dc.subject | Baicalein | |
| dc.subject | Chromatin Damage | |
| dc.subject | Cyopreservation | |
| dc.subject | Oxidative Stress | |
| dc.subject | Ram Semen | |
| dc.title | Cryopreservation of ram semen: baicalein efficiency on oxidative stress, chromatin integrity, viability and motility post thaw | |
| dc.type | Article |
