Effects of Dietary Anise (Pimpinella anisum L.) Oil on Growth Performance, Blood Parameters and Muscle Nutrient Content of Nile Tilapia (Oreochromis niloticus)

Tok, Serkan; Öz, Mustafa; Dikel, Suat

Effects of Dietary Anise (Pimpinella anisum L.) Oil on Growth Performance, Blood Parameters and Muscle Nutrient Content of Nile Tilapia (Oreochromis niloticus)

dc.authorid	0000-0001-5264-7103
dc.contributor.author	Tok, Serkan
dc.contributor.author	Öz, Mustafa
dc.contributor.author	Dikel, Suat
dc.date.accessioned	2025-07-09T08:16:32Z
dc.date.available	2025-07-09T08:16:32Z
dc.date.issued	2025
dc.department	Sağlık Bilimleri Enstitüsü
dc.description.abstract	Objectives: This study aimed to investigate the effects of dietary Pimpinella anisum (anise) oil supplementation on growth performance, haematological and biochemical parameters, antioxidant enzyme activities and tissue histopathology in Nile tilapia (Oreochromis niloticus). The goal was to determine the optimal inclusion level that promotes health benefits without inducing toxicity. Methodology: A total of 240 juvenile Nile tilapia were randomly distributed into four groups: control (0.00%) and three treatments receiving anise oil at 0.10%, 0.20% and 0.30% for 8 weeks. Growth performance (weight gain, feed conversion ratio), haematological (RBC, Hb, WBC) and biochemical parameters (ALT, AST, glucose, lipids), oxidative stress markers (SOD, CAT, GPx) and histopathological changes in gill, liver, kidney and muscle tissues were evaluated. Results: Anise oil supplementation at 0.10% and 0.20% significantly improved growth performance, haematological indices and antioxidant enzyme activities (p < 0.05) compared to the control group. However, fish fed 0.30% anise oil showed elevated liver enzymes, glucose and lipid levels, along with moderate tissue damage, indicating potential adverse effects at higher doses. Conclusions: Dietary inclusion of 0.10% to 0.20% anise oil enhances the growth, physiological health and antioxidant defence in Nile tilapia. Nevertheless, 0.30% inclusion may impair metabolic balance and tissue integrity. Anise oil represents a promising natural feed additive for sustainable aquaculture when used at optimal levels.
dc.identifier.doi	10.1002/vms3.70443
dc.identifier.issn	20531095
dc.identifier.issue	4
dc.identifier.pmid	40454886
dc.identifier.scopus	105007539685
dc.identifier.uri	https://dx.doi.org/10.1002/vms3.70443
dc.identifier.uri	https://hdl.handle.net/20.500.12451/13217
dc.identifier.volume	11
dc.identifier.wos	WOS:001500313600001
dc.identifier.wosquality	Q2
dc.indekslendigikaynak	PubMed
dc.indekslendigikaynak	Scopus
dc.indekslendigikaynak	Web of Science
dc.institutionauthor	Tok, Serkan
dc.institutionauthor	Öz, Mustafa
dc.institutionauthorid	0000-0001-5264-7103
dc.language.iso	en
dc.publisher	John Wiley and Sons Inc
dc.relation.ispartof	Veterinary Medicine and Science
dc.relation.publicationcategory	Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	Anise Oil
dc.subject	Antioxidant Properties
dc.subject	Blood Parameters
dc.subject	Growth Performance
dc.subject	Nile Tilapia
dc.title	Effects of Dietary Anise (Pimpinella anisum L.) Oil on Growth Performance, Blood Parameters and Muscle Nutrient Content of Nile Tilapia (Oreochromis niloticus)
dc.type	Article