Microcontact imprinted plasmonic nanosensors: Powerful tools in the detection of salmonella paratyphi

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dc.authoridyilmaz, erkut -- 0000-0002-1217-5225;
dc.contributor.authorPerçin, Işık
dc.contributor.authorİdil, Neslihan
dc.contributor.authorBakhshpour, Monireh
dc.contributor.authorYılmaz, Erkut
dc.contributor.authorMattiasson, Bo
dc.contributor.authorDenizli, Adil
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:28:44Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:28:44Z
dc.date.issued2017
dc.departmentFen-Edebiyat Fakültesi
dc.description.abstractIdentification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time Salmonella paratyphi detection was performed within the range of 2.5 x 10(6)-15 x 10(6) CFU/mL. Selectivity of the prepared sensors was examined by using competing bacterial strains such as Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The imprinting efficiency of the prepared sensor system was determined by evaluating the responses of the SPR chips prepared with both molecularly imprinted polymers (MIPs) and non-imprinted polymers (NIPs). Real sample experiments were performed with apple juice. The recognition of Salmonella paratyphi was achieved using these SPR sensor with a detection limit of 1.4 x 10(6) CFU/mL. In conclusion, SPR sensor has the potential to serve as an excellent candidate for monitoring Salmonella paratyphi in food supplies or contaminated water and clearly makes it possible to develop rapid and appropriate control strategies.
dc.identifier.doi10.3390/s17061375
dc.identifier.issn1424-8220
dc.identifier.issue6en_US
dc.identifier.pmid28608810
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/s17061375
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6079
dc.identifier.volume17en_US
dc.identifier.wosWOS:000404553900191
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMDPI
dc.relation.ispartofSENSORS
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMicrocontact Imprinting
dc.subjectSPR Biosensor
dc.subjectN-Methacryloyl-L-Histidine Methyl Ester
dc.subjectSalmonella Paratyphi
dc.titleMicrocontact imprinted plasmonic nanosensors: Powerful tools in the detection of salmonella paratyphi
dc.typeArticle

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