Sorption of DNA by diatomite-Zn (Ii) embedded supermacroporous monolithic p(HEMA) cryogels

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dc.contributor.authorÖzcan Tozak, Kabil
dc.contributor.authorErzengin, Mahmut
dc.contributor.authorSargın, İdris
dc.contributor.authorÜnlü, Nuri
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T08:22:54Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T08:22:54Z
dc.date.issued2013
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractIn this study, the DNA sorption performance of diatomite-Zn(II) embedded supermacroporous monolithic p(HEMA) cryogels were investigated for the purpose of designing a novel adsorbent that can be utilized for DNA purification, separation and immunoadsorption studies such as removal of anti-dsDNA antibodies from systemic lupus erythematosus (SLE) patient plasma. Poly(2-hydroxyethyl methacrylate) [p(HEMA)]-based monolithic cryogel column embedded with Zn2+-diatomite particles was prepared by free radical cryocopolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N'-methylene-bis-acrylamide (MBAAm). The polymerization reaction was initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. After thawing, the monolithic composite cryogels were used for affinity sorption and then subsequent desorption of DNA molecules from aqueous solutions. Diatomite (DA) particles were characterized by XRF and BET method. The characterization of composite cryogel was done through SEM imaging. The effects of pH of the solution, initial DNA concentration, ionic strength, temperature and flow rates on adsorption were investigated to determine the optimum conditions for adsorption/desorption experiments. The particle embedding procedure was shown to yield significantly enhanced adsorption of DNA on the adsorbent. Furthermore, considering its excellent bio-compatibility, p(HEMA) cryogels are promising a candidate for further DNA sorption studies.
dc.identifier.endpage680en_US
dc.identifier.issn1611-2156
dc.identifier.scopusqualityQ1
dc.identifier.startpage670en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12451/2667
dc.identifier.volume12en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.relation.ispartofEXCLI Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectComposite Cryogel
dc.subjectDiatomite
dc.subjectImmobilized Metal Affinity Chromatography
dc.subjectParticle Embedding
dc.titleSorption of DNA by diatomite-Zn (Ii) embedded supermacroporous monolithic p(HEMA) cryogels
dc.typeArticle

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