In vitro effects of some antibıotics on enzyme activity of carbonic anhydrase from bovine erythrocytes

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dc.contributor.authorArslan, Mikail
dc.contributor.authorBeyaztaş, Serap
dc.contributor.authorErzengin, Mahmut
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:15:06Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:15:06Z
dc.date.issued2011
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractThe purpose of this study was to investigate the in vitro effects of six commonly used veterinary medical drugs (Killoxacin (TM), Gentavet (TM), Geosol (TM), Primoxal (TM), Lypectin (TM) and Penoksal-la (TM)) on erythrocyte bovine carbonic anhydrase (CA, EC 4.2.1.1) activity. The enzyme was purified by affinity chromatography, and the purity was confirmed by SDS-PAGE. Inhibition or activation effects of six different antibiotics on the purified enzyme were determined using the CO2-hydratase activity method. IC50 values of the drugs that caused inhibition were determined by means of Activity % vs [Inhibitor] diagrams. The concentrations of Killoxacin (TM), Gentavet (TM), Geosol (TM), Primoxal (TM) and Lypectin (TM) that inhibited 50% of the enzymatic activity were 9.25, 3.79, 0.72, 6.59 and 2.12 mM, respectively. On the other hand, the enzyme activity was increased by Penoksal-la (TM).
dc.identifier.endpage445en_US
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue2Aen_US
dc.identifier.scopusqualityN/A
dc.identifier.startpage439en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4284
dc.identifier.volume20en_US
dc.identifier.wosWOS:000289040400004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherParlar Scientific Publications
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBovine carbonic anhydrase
dc.subjectIn Vitro
dc.subjectInhibition
dc.subjectVeterinary Medical Drugs
dc.subjectAffinity Chromatography
dc.subjectPurification
dc.titleIn vitro effects of some antibıotics on enzyme activity of carbonic anhydrase from bovine erythrocytes
dc.typeArticle

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