In vitro effects of some antibıotics on enzyme activity of carbonic anhydrase from bovine erythrocytes
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Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The purpose of this study was to investigate the in vitro effects of six commonly used veterinary medical drugs (Killoxacin (TM), Gentavet (TM), Geosol (TM), Primoxal (TM), Lypectin (TM) and Penoksal-la (TM)) on erythrocyte bovine carbonic anhydrase (CA, EC 4.2.1.1) activity. The enzyme was purified by affinity chromatography, and the purity was confirmed by SDS-PAGE. Inhibition or activation effects of six different antibiotics on the purified enzyme were determined using the CO2-hydratase activity method. IC50 values of the drugs that caused inhibition were determined by means of Activity % vs [Inhibitor] diagrams. The concentrations of Killoxacin (TM), Gentavet (TM), Geosol (TM), Primoxal (TM) and Lypectin (TM) that inhibited 50% of the enzymatic activity were 9.25, 3.79, 0.72, 6.59 and 2.12 mM, respectively. On the other hand, the enzyme activity was increased by Penoksal-la (TM).
Açıklama
Anahtar Kelimeler
Bovine carbonic anhydrase, In Vitro, Inhibition, Veterinary Medical Drugs, Affinity Chromatography, Purification
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
20
Sayı
2A
