Purification and characterization of organic solvent-tolerant lipase from Streptomyces sp. OC119-7 for biodiesel production

Basit Öğe Kaydı
dc.contributor.authorAyaz, Berk
dc.contributor.authorUğur, Aysel
dc.contributor.authorBoran, Rukiye
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:27:02Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:27:02Z
dc.date.issued2015
dc.departmentSağlık Hizmetleri Meslek Yüksekokulu
dc.description.abstractThis study determined the lipolytic activity of Streptomyces isolates and then purified and characterized the lipase obtained from Streptornyce.s sp. OC 119-7, the isolate demonstrated to have the high lipolytic activity. Ammonium sulfate precipitation and gel filtration chromatography were used to purify the extracellular alkaline lipase obtained from StTeptornyces sp. OC 119-7, and resulted in 5.52-fold purification with 68.055 U/mg specific activity. The enzyme showed optimal activity at pH 8.0 and 50 degrees C, with stability in a temperature range of 40-60 degrees C and at pHs of >= 7. Enzyme activity was enhanced by the presence of Ca2+ and Mg2+ and inhibited by the presence of Mn2+, Co2+, Cu2+, Zn2+, K+, Na+ and PMSF. OC 119-7 lipase displayed stability against surfactants and organic solvents. Lip0C 119-7 catalyzed transesterification of olive oil with methanol, suggesting that this lipase may be a potential enzymatic catalyst for biodiesel production. (C) 2014 Elsevier Ltd. All rights reserved.
dc.identifier.doi10.1016/j.bcab.2014.11.007
dc.identifier.endpage108en_US
dc.identifier.issn1878-8181
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage103en_US
dc.identifier.urihttps://doi.org/10.1016/j.bcab.2014.11.007
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5772
dc.identifier.volume4en_US
dc.identifier.wosWOS:000361111000016
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofBiocatalysis and Agricultural Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectBiodiesel
dc.subjectEnzyme Characterization
dc.subjectLipase
dc.subjectStreptomyces
dc.titlePurification and characterization of organic solvent-tolerant lipase from Streptomyces sp. OC119-7 for biodiesel production
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
ayaz-berk-2015.pdf
Boyut:
606.04 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu