Anti-proliferative effects of gold nanoparticles functionalized with Semaphorin 3F

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dc.authoridTan, Gamze -- 0000-0002-2755-1756
dc.contributor.authorTan, Gamze
dc.contributor.authorOnur, Mehmet Ali
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:15:23Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:15:23Z
dc.date.issued2017
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractThe new vessel formations play a vital role in growth and spread of cancer. Current anti-angiogenic therapies, predominantly based on vascular endothelial growth factor (VEGF) inhibition, can inhibit vascular development; however, they are usually ineffective against the primary tumor occurrence. The aim of this study was to assess anti-angiogenic effects of gold nanoparticles (AuNPs) functionalized with Semaphorin (Sema) 3F protein. The polyethylene glycol (PEG)-coated AuNPs were covalently functionalized with Sema 3F and labeled with the TAMRA fluorescent dye. The effect of the NPs on human umbilical vein endothelial cells (HUVECs) is probed in the way of internalization and viability assays. AuNP-Sema 3F bioconjugates showed great endothelial cell uptake. AuNP-Sema 3F bioconjugates reduced VEGF(165)-induced endothelial cell proliferation more effectively than Sema 3F alone, suggesting that the therapeutic effects of Sema 3F can be improved by conjugation to AuNPs. Also, no significant toxicity effect was induced by bioconjugates. This is the first study that reports a covalent binding of full length Sema 3F to NPs. The exogenously administration of Sema 3F, which has both anti-angiogenic and antitumoral activity, to tumor vasculature via a carrying platform may not only lead to more effective antiangiogenic treatment but also may make current approach more applicable in clinical use like drug delivery system.
dc.description.sponsorshipHacettepe University Scientific Research Projects Coordination Unit [010BIYP02604003, 014BIYP604004]
dc.description.sponsorshipThis work was supported by the Hacettepe University Scientific Research Projects Coordination Unit (project number: 010BIYP02604003 partially, 014BIYP604004).
dc.identifier.doi10.1007/s11051-017-3967-7
dc.identifier.issn1388-0764
dc.identifier.issn1572-896X
dc.identifier.issue8en_US
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s11051-017-3967-7
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4368
dc.identifier.volume19en_US
dc.identifier.wosWOS:000410849000003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofJournal of Nanoparticle Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAngiogenesis
dc.subjectBioconjugation
dc.subjectGold Nanoparticles
dc.subjectHUVEC
dc.subjectSemaphorin 3F
dc.subjectVEGF
dc.titleAnti-proliferative effects of gold nanoparticles functionalized with Semaphorin 3F
dc.typeArticle

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