A histological and biochemical study of the protective role of hesperidin in testicular ischemia-reperfusion injury
| dc.authorid | 0000-0002-8457-9448 | |
| dc.authorid | 0000-0001-5573-4923 | |
| dc.authorid | 0000-0001-8863-817X | |
| dc.contributor.author | Akaras, Nurhan | |
| dc.contributor.author | Şimşek, Hasan | |
| dc.contributor.author | Ordu, Melike | |
| dc.date.accessioned | 2023-05-16T07:21:28Z | |
| dc.date.available | 2023-05-16T07:21:28Z | |
| dc.date.issued | 2023 | |
| dc.department | Tıp Fakültesi | |
| dc.description.abstract | This study aimed to investigate the possible effects of hesperidin on ischemia-reperfusion (IR) injury applied to rat testis. Methods: Twenty-eight Wistar albino rats were used and divided into four groups of seven each. Group 1: Sham surgery was performed on the right testis. Group 2: Hesperidin 100 mg/kg was administered intraperitoneally to rats. Group 3: After 1 h of ischemia and 4 h of reperfusion, the testicles were removed. Group 4: 100 mg/kg hesperidin was given 30 min before reperfusion. Biochemical, immunohistochemical, and histopathological analyzes were performed on testicles obtained from each group. Results: Total oxidant status (TOS) and oxidative stress index (OSI) levels increased significantly in the IR and IR-He groups (respectively, p=0.016, p=0.041; p=0.01, and p=0.024). TOS and OSI values in the hesperidin group decreased, although not statistically significant, compared to the IR group. Tumor necrosis factor-alpha (TNF-?) and nuclear factor kappa B (NF-kB) values were decreased in the hesperidin group compared to the IR group, although it was not statistically significant. Caspase-3 levels in testicular tissue were significantly increased in the IR group compared to the hesperidin group (p<0.05). While there were degenerative changes in the testicular tissue in the IR groups, a decrease in bleeding, congestion, edema, and degenerative changes was observed in the hesperidin-administered groups. Conclusion: Hesperidin reduced oxidative stress (decreased total oxidant level and OSI), inflammation (TNF-?), and apoptosis (NF-kB and caspase-3). According to these results, it was observed that hesperidin application had a protective effect on IR injury. | |
| dc.identifier.doi | 10.14744/ijmb.2022.39306 | |
| dc.identifier.endpage | 27 | en_US |
| dc.identifier.issn | 2587-2362 | |
| dc.identifier.issn | 2618-642X | |
| dc.identifier.issue | 1 | en_US |
| dc.identifier.scopusquality | Q4 | |
| dc.identifier.startpage | 21 | en_US |
| dc.identifier.uri | https:/dx.doi.org/10.14744/ijmb.2022.39306 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12451/10794 | |
| dc.identifier.volume | 6 | en_US |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | TR-Dizin | |
| dc.language.iso | en | |
| dc.publisher | Ali Cangül | |
| dc.relation.ispartof | International Journal of Medical Biochemistry | |
| dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | Apoptosis | |
| dc.subject | Hesperidin | |
| dc.subject | Ischemia-reperfusion Injury | |
| dc.subject | Testis | |
| dc.title | A histological and biochemical study of the protective role of hesperidin in testicular ischemia-reperfusion injury | |
| dc.type | Article |
