Alternative screening method for analyzing the water samples through an electrical microfluidics chip with classical microbiological assay comparison of P. aeruginosa

dc.authorid0000-0001-9647-0338
dc.contributor.authorBilican, İsmail
dc.contributor.authorBahadır, Tolga
dc.contributor.authorBilgin, Kemal
dc.contributor.authorGüler, Mustafa Tahsin
dc.date.accessioned2020-09-07T06:23:27Z
dc.date.available2020-09-07T06:23:27Z
dc.date.issued2020
dc.departmentRektörlük
dc.description*Bilican, İsmail ( Aksaray, Yazar ) *Bahadır, Tolga ( Aksaray, Yazar )
dc.description.abstractPseudomonas aeruginosa is a pathogenic bacterium in fresh water supplies that creates a risk for public health. Microbiological analysis of drinking water samples is time consuming and requires qualified personnel. Here we offer a screening system for rapid analysis of spring water that has the potential to be turned into a point-of-need system by means of simple mechanism. The test, which takes 1 h to complete, electrically interrogates the particles through a microfluidic chip suspended in the water sample. We tested the platform using water samples with micro beads and water samples spiked with P. aeruginosa at various concentrations. The mono disperse micro beads were used to evaluate the performance of the system. The results were verified by the gold standard membrane filtration method, which yielded a positive test result only for the P. aeruginosa spiked samples. Detection of 0–11 k bacteria in 30 ?L samples was successfully completed in 1 h and compared with a conventional microbiological method. The presented method is a good candidate for a rapid, on-site, screening test that can result in a significant reduction in cost and analysis time compared to microbiological analyses routinely used in practice.
dc.identifier.doi10.1016/j.talanta.2020.121293
dc.identifier.endpage-en_US
dc.identifier.issn0039-9140
dc.identifier.issue-en_US
dc.identifier.pmid32887035
dc.identifier.scopusqualityQ1
dc.identifier.startpage-en_US
dc.identifier.urihttps:/dx.doi.org/10.1016/j.talanta.2020.121293
dc.identifier.urihttps://hdl.handle.net/20.500.12451/7655
dc.identifier.volume219en_US
dc.identifier.wosWOS:000568750500006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectBacteria
dc.subjectElectrical Detection
dc.subjectMicrofluidic Chip
dc.subjectP. Aeruginosa
dc.subjectWater Analysis
dc.titleAlternative screening method for analyzing the water samples through an electrical microfluidics chip with classical microbiological assay comparison of P. aeruginosa
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
bilican-ismail-2020.pdf
Boyut:
9 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama: