Comparative study of ASNase immobilization on tannic acid-modified magnetic Fe3O4/SBA-15 nanoparticles to enhance stability and reusability

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dc.authorid0000-0002-0198-2443
dc.authorid0000-0003-1271-0555
dc.authorid0000-0001-6080-229X
dc.contributor.authorNoma, Samir Abbas Ali
dc.contributor.authorUlu, Ahmet
dc.contributor.authorAcet, Ömür
dc.contributor.authorSanz, Raúl
dc.contributor.authorSanz-Pérez, Eloy S.
dc.contributor.authorOdabaşı, Mehmet
dc.contributor.authorAteş, Burhan
dc.date.accessioned2020-04-07T10:33:03Z
dc.date.available2020-04-07T10:33:03Z
dc.date.issued2020
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description*Acet, Ömür ( Aksaray, Yazar ) *Odabaşı, Mehmet ( Aksaray, Yazar )
dc.description.abstractIn this work, l-asparaginase was immobilized on tannic acid-modified magnetic mesoporous particles. In brief, Fe3O4/SBA-15/tannic acid magnetic particles were synthesized, and their structures and morphologies were fully characterized using various methods. The properties of the free and immobilized enzyme were examined in terms of pH, temperature, thermal stability, storage stability, and reusability. Moreover, the effects of metal ions, inhibitors and organic solvents on the activity of the immobilized enzyme were investigated. Compared to the free enzyme, the immobilized enzyme possessed better tolerance to changes in ambient temperature and pH. Additionally, thermal incubation results showed that the free enzyme lost its activity, while the immobilized enzyme exhibited the opposite behavior. Most strikingly, the immobilized l-asparaginase exhibited a high degree of activity (70%) after being reused 16 times while also demonstrating 71% and 63% storage stability of the initial activity even after 28 days at 4 °C and room temperature, respectively. Together with these results, l-asparaginase was successfully immobilized upon Fe3O4/SBA-15/tannic acid magnetic nanoparticles with improved stability properties. This support holds great potential and opens up a novel perspective for growing applications.
dc.identifier.doi10.1039/d0nj00127a
dc.identifier.endpage4451en_US
dc.identifier.issn1144-0546
dc.identifier.issue11en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage4440en_US
dc.identifier.urihttps:/dx.doi.org/10.1039/d0nj00127a
dc.identifier.urihttps://hdl.handle.net/20.500.12451/7496
dc.identifier.volume44en_US
dc.identifier.wosWOS:000521102000024
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.relation.ispartofNew Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEnzyme Activity
dc.subjectFlavonoids
dc.subjectIron Oxides
dc.subjectMagnetism
dc.subjectMagnetite
dc.subjectMetal Ions
dc.subjectMetals
dc.subjectReusability
dc.subjectStability
dc.subjectTannins
dc.titleComparative study of ASNase immobilization on tannic acid-modified magnetic Fe3O4/SBA-15 nanoparticles to enhance stability and reusability
dc.typeArticle

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