How important is group size in selecting rats for experimental studies? evolution with zootechnical and biochemical findings
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Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, which was conducted to investigate the growth performances of male Sprague-Dawley rats grown in different group sizes and changes that occurred in their oxidative statuses, 60 male 4-week-old Sprague-Dawley rats were used. Two different cages with different dimensions were used, and 4 rats were put into each of the small cages, while 6 were put into each of the large cages. The trial was carried out with 6 replications. The rats were weighed each week for one month on the same day and at the same hour, and the average cage values were recorded. It was determined that the live weights showed a regular increase in both groups, and the growth performances did not differ based on group size. Group size was highly effective on the antioxidant components of the rats, and growing the rats in more crowded environments led to oxidative stress by changing their Total Antioxidant Capacity (TAC) (p<0.05), Total Oxidant Capacity (TOC) (p<0.05), Nitric Oxide (NO) (p<0.001), glutathione (GSH) (p<0.0001) and malondialdehyde (MDA) (p<0.001) levels. In the light of the data obtained, it was concluded that, when dense rat breeding is carried out to achieve more production from a unit area, even though no changes occur in the morphological structures of the animals, their oxidant balance is disrupted. This prevents obtaining accurate and reliable data in scientific studies. Consequently, it was determined that group size is a criterion that needs to be kept in mind in selection of rats to be used in scientific studies, and the appropriate group size should be provided for these animals.
Açıklama
Anahtar Kelimeler
Group Size, Sprague-Dawley, Growth Performance, Oxidative and Nitrosative Stress
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
31
Sayı
6A
