Characterization of chitin and description of its antimicrobial properties obtained from Cydalima perspectalis adults

dc.authorid0000-0002-3018-6905
dc.authorid0000-0002-2657-9263
dc.authorid0000-0003-1047-8350
dc.authorid0000-0003-0728-0968
dc.contributor.authorKılcı, Leyla
dc.contributor.authorAltun, Nurver
dc.contributor.authorKaraoğlu, Şengül Alpay
dc.contributor.authorKaraduman Yeşildal, Tuğçe
dc.date.accessioned2024-07-16T11:30:11Z
dc.date.available2024-07-16T11:30:11Z
dc.date.issued2024
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractChitin is the most abundant biopolymer group after cellulose and forms the exoskeleton of arthropods, the largest animal group. The morphology of chitin differs between and within species. In this study, we determined the physicochemical and biological activity of chitin samples obtained from different body parts of the boxwood moth Cydalima perspectalis for its application as a biotechnological material. The collected chitin samples were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and elemental analysis. FTIR confirms that the isolation of chitin is successful. The SEM results showed that the surface morphology of the obtained chitins was both fibrous and porous and had a rough surface. As a result of the elemental analysis, the %N values of chitins were calculated as 6.60 on average, and the values were shown to be close to each other. We also investigated the biocompatibility and antimicrobial properties of these chitin samples. We used L929 (mouse fibroblast) cells to perform indirect cytotoxicity experiments and investigated their viability by performing the MTT assay. Our findings showed that the samples had no cytotoxic effect on the L929 cells at 24, 48, and 72 h. The cytotoxic study showed that Escherichia coli, Bacillus subtilis, Staphylococcus aureus bacteria, and Candida albican fungi adhered to chitin surfaces regarding biofilm production. The chitin contents were determined as 21.02% for the head, 5.74% for the body, 32.22% for the wing, 33.53% for the legs, and 2.65% for the pupal shell. Chitin is a material with high potential for use in various fields. Our findings suggested that Cydalima perspectalis can be used as an alternative source of chitin in biomedical application.
dc.identifier.doi10.1007/s00289-024-05381-z
dc.identifier.issn0170-0839
dc.identifier.scopusqualityQ1
dc.identifier.urihttps:/dx.doi.org/10.1007/s00289-024-05381-z
dc.identifier.urihttps://hdl.handle.net/20.500.12451/12130
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer Science and Business Media Deutschland GmbH
dc.relation.ispartofPolymer Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCharacterization
dc.subjectChitin
dc.subjectCydalima Perspectalis
dc.subjectLepidoptera
dc.titleCharacterization of chitin and description of its antimicrobial properties obtained from Cydalima perspectalis adults
dc.typeArticle

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