Impact of DBP on histology and expression of HSP 70 in gill and liver tissue of Cyprinus carpio

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dc.authoridAgus, Hızlan Hincal -- 0000-0002-0252-9501;
dc.contributor.authorAgus, Hızlan H.
dc.contributor.authorErkmen, Belda
dc.contributor.authorSümer, Sibel
dc.contributor.authorSepici-Dincel, Aylin
dc.contributor.authorErkoç, Figen
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:28:53Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:28:53Z
dc.date.issued2015
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractDi-n-butyl phthalate (DBP) widely used plasticizer in the plastic industry, affects regulation of the endocrine system and causes toxicity in animals. In the present study, the aim was to study the toxic effects/damages of DBP exposure using Hsp70 levels and histopathological changes in Carp liver and gill. Hsp70 expression levels were assessed as specific biomarker of in vivo ecotoxicological stress. Carp (Cyprinus carpio) were exposed to sub-lethal concentration of DBP (di-n-butyl phthalate, 1 mg/L) for 4, 24 and 96 h. Gill and liver tissues were evaluated histopathologically and RNA quantifications for Hsp70 expression levels were carried out using a two-step real-time RT-PCR. In liver, a rapid but non-significant increase in mRNA levels in the first 4 h was observed. mRNA levels significantly increased up to 2-3 fold after 24 and 96 h (p < 0.05). However, irregular mRNA level changes were also recorded: Gill specific and time-dependent regulation of Hsp70 expression were 4-5 fold inhibition after 4 and 24 h (p < 0.05), then increased up to 4 fold after 96 h (p < 0.05). Histopathological findings support altered transcription results as: Epithelial lifting, hyperplasia, fusion of secondary lamellae, telangiectasis, passive hyperemia and hydropic degeneration. Significant alterations of Hsp70 levels were likely due to a tissue specific response against chemical stress, cellular damage and lesions due to DBP. Carp was found to be a suitable experimental model for toxicology, and Hsp70 mRNA levels are reliable, specific biomarkers.
dc.description.sponsorshipGazi University [04/2012-11]; Turkish Scientific and Technological Research Council of Turkey [212T185]
dc.description.sponsorshipThe present study was partially supported by the: Gazi University, Research Fund, through project contract no: 04/2012-11 and The Turkish Scientific and Technological Research Council of Turkey, contract no: 212T185. Special thanks to Pinar Arslan, graduate student from Ankara University for her help with the experimentation.
dc.identifier.doi10.1007/s11033-015-3920-8
dc.identifier.endpage1417en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue9en_US
dc.identifier.pmid26311152
dc.identifier.scopusqualityQ2
dc.identifier.startpage1409en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-015-3920-8
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6099
dc.identifier.volume42en_US
dc.identifier.wosWOS:000360882900007
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMoleculer Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPhthalate
dc.subjectHistopathology
dc.subjectEcotoxicology
dc.subjectBiomarkers
dc.subjectHsp70 expression
dc.subjectCyprinus Carpio
dc.titleImpact of DBP on histology and expression of HSP 70 in gill and liver tissue of Cyprinus carpio
dc.typeArticle

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