Cardioprotective effects of fetai Icidney-derived mesencliymai stem ceiis on doxorubicin-induced cardiotoxicity in rats
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Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Polskie Towarzystwo Nauk Weterynaryjnych
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Cardiotoxicity is one of the most common side effects of doxorubicin (DOX), a chemotlierapy drug used in tlie treatment of many carcinomas. In recent years, stem-cell therapies have been successfully used to prevent cardiotoxicity. This study investigated the efficacy of intraperitoneally administered fetal kidney-derived mesenchymal stem cells (FKD-MSCs) in preventing DOX-induced cardiotoxicity in rats. For this purpose, thirty rats were randomly divided into three groups: control, DOX and mesenchymal stem cell (MSG) groups. Adriamycin was injected as a single dose via the tail vein in the DOX and MSG groups in order to induce cardiotoxicity. FKD-MSG was applied to the MSG group by the intraperitoneal route after cardiotoxicity had been established. Then the rats were euthanized, and routine histological procedures were performed on their hearts. H&E and Masson's stains were used for histopathology. Gardiac Troponin-T and I (cTnT, cTnl), Gaspase-3 and BGL-XL antibodies were used for immunohistochemistry. Vacuoles, edema, degeneration and necrosis were observed histopathologically mostly in the DOX group. Lesions in the control and MSG groups were less severe. Fibrosis in the control and MSG groups was milder. cTnT and cTnl immunopositive staining was most commonly seen in the control group, followed by the MSG group. Immunohistochemical staining by Gaspase-3 and BGL-XL showed that their expressions in the MSG group were statistically similar to those in the control group. Accordingly, it was concluded that the intraperitoneal application of MSG had a positive effect on histopathological findings, fibrosis, immunohistochemistry, especially apoptosis, neovascularization, and anti-apoptotic development, whereas troponin levels were not found to be therapeutic.
Açıklama
Anahtar Kelimeler
Apoptosis, Cardiotoxicity, Doxorubicin, Stem Cell, Troponin
Kaynak
Medycyna Weterynaryjna
WoS Q Değeri
N/A
Scopus Q Değeri
Q4
Cilt
78
Sayı
2
