Cholesterol-loaded cyclodextrin plus trehalose improves quality of frozen-thawed ram sperm

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dc.contributor.authorİnanç, Muhammed Enes
dc.contributor.authorGüngör, Şükrü
dc.contributor.authorÖztürk, Caner
dc.contributor.authorKorkmaz, Fırat
dc.contributor.authorBaştan, İlktan
dc.contributor.authorÇil, Beste
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:16:37Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:16:37Z
dc.date.issued2019
dc.departmentVeteriner Fakültesi
dc.description.abstractThe objective of this study was to determine effects of supplementing 'Eris-based semen extenders with either cholesterol-loaded cyclodextrin (CLC) or 7-dehydrocholesterol loaded cyclodextrin (7-DCLC) plus trehalose (T) for cryopreservation of ram semen. Semen was collected with an artificial vagina from five Merino rams (2-3 years of age) during the non-breeding season. Ejaculates were pooled, divided into eight equal portions, diluted with a standard Tris-based extender containing: no additive (control); T (50 mM); or T (50 mM) + 1.5, 2.5 or 3.5 mg of either 7-DCLC or CLC. Semen was chilled from 37 degrees C to 4 degrees C, placed in 0.25 ml French straws, held 5 cm above liquid nitrogen for 12 minutes, then plunged into liquid nitrogen. After thawing, a computer-aided semen analyzer system (CASA) was used to assess motility, whereas plasma membrane and acrosome integrity (PMAI) and high mitochondrial membrane potential (HMMP) were assessed with flow cytometry. Sperm supplemented with 2.5 mg and 3.5 mg CLC + T had the highest (P < 0.05) total and progressive motility (65.2 +/- 4.7 and 19.0 +/- 1.0% respectively, mean +/- SEM), albeit with no significant differences from sperm with 1.5 or 3.5 mg Cl,C + T. Sperm with 2.5 mg CLC + T had the highest (P < 0.05) PMAI (59.3%; not different from 3.5 mg CLC + T) and highest (P < 0.05) HMMP (64.6%; not different from 1.5 or 3.5 mg CLC + T). The lowest ALH value, 2.8 +/- 0.3 pm was in the 2.5 mg 7-DCLC + T group; otherwise, there were no significant differences among groups for any other CASA end point. In conclusion, adding CLC + T to a tris-based extender optimized quality of frozen-thawed ram semen. Therefore, extenders including CLC + T have potential to improve quality of frozen-thawed ram sperm.
dc.identifier.doi10.17221/146/2018-VETMED
dc.identifier.endpage124en_US
dc.identifier.issn0375-8427
dc.identifier.issn1805-9392
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ3
dc.identifier.startpage118en_US
dc.identifier.urihttps://doi.org/10.17221/146/2018-VETMED
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4626
dc.identifier.volume64en_US
dc.identifier.wosWOS:000461825100003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherCzech Academy of Agricultural Sciences
dc.relation.ispartofVeterinární medicína
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject7-dehydrocholesterol
dc.subjectSemen Cryopreservation
dc.subjectComputer-Assisted Semen Analyzer
dc.subjectFlow Cytometry
dc.titleCholesterol-loaded cyclodextrin plus trehalose improves quality of frozen-thawed ram sperm
dc.typeArticle

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