Alteration of the fecal microbiota and serum metabolite profiles in dogs with idiopathic inflammatory bowel disease

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dc.authoridSuchodolski, Jan -- 0000-0002-2176-6932
dc.contributor.authorMinamoto, Yasushi
dc.contributor.authorOtoni, Cristiane C.
dc.contributor.authorSteelman, Samantha M.
dc.contributor.authorBüyükleblebici, Olga
dc.contributor.authorSteiner, Jorg M.
dc.contributor.authorJergens, Albert E.
dc.contributor.authorSuchodolski, Jan S.
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:26:17Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:26:17Z
dc.date.issued2015
dc.departmentVeteriner Fakültesi
dc.description.abstractIdiopathic inflammatory bowel disease (IBD) is a common cause of chronic gastrointestinal (GI) disease in dogs. The combination of an underlying host genetic susceptibility, an intestinal dysbiosis, and dietary/environmental factors are suspected as main contributing factors in the pathogenesis of canine IBD. However, actual mechanisms of the host-microbe interactions remain elusive. The aim of this study was to compare the fecal microbiota and serum metabolite profiles between healthy dogs (n = 10) and dogs with IBD before and after 3 weeks of medical therapy (n = 12). Fecal microbiota and metabolite profiles were characterized by 454-pyrosequencing of 16 S rRNA genes and by an untargeted metabolomics approach, respectively. Significantly lower bacterial diversity and distinct microbial communities were observed in dogs with IBD compared to the healthy control dogs. While Gammaproteobacteria were overrepresented, Erysipelotrichia, Clostridia, and Bacteroidia were underrepresented in dogs with IBD. The functional gene content was predicted from the 16 S rRNA gene data using PICRUSt, and revealed overrepresented bacterial secretion system and transcription factors, and underrepresented amino acid metabolism in dogs with IBD. The serum metabolites 3-hydroxybutyrate, hexuronic acid, ribose, and gluconic acid lactone were significantly more abundant in dogs with IBD. Although a clinical improvement was observed after medical therapy in all dogs with IBD, this was not accompanied by significant changes in the fecal microbiota or in serum metabolite profiles. These results suggest the presence of oxidative stress and a functional alteration of the GI microbiota in dogs with IBD, which persisted even in the face of a clinical response to medical therapy.
dc.description.sponsorshipComparative Gastroenterology Society/Waltham Research Grant
dc.description.sponsorshipThis study was supported by the Comparative Gastroenterology Society/Waltham Research Grant.
dc.identifier.doi10.1080/19490976.2014.997612
dc.identifier.endpage47en_US
dc.identifier.issn1949-0976
dc.identifier.issn1949-0984
dc.identifier.issue1en_US
dc.identifier.pmid25531678
dc.identifier.scopusqualityQ1
dc.identifier.startpage33en_US
dc.identifier.urihttps://doi.org/10.1080/19490976.2014.997612
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5519
dc.identifier.volume6en_US
dc.identifier.wosWOS:000457442100004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofGut Mıcrobes
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDog
dc.subjectDysbiosis
dc.subjectFeces
dc.subjectIBD
dc.subjectMicrobiome
dc.subjectMetabolomics
dc.subjectFaecalibacterium
dc.titleAlteration of the fecal microbiota and serum metabolite profiles in dogs with idiopathic inflammatory bowel disease
dc.typeArticle

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