Therapeutic potential of Laurus nobilis extract by experimental and computational approaches: phenolic content and bioactivities for antioxidant, antidiabetic, and anticholinergic properties

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dc.contributor.authorAltın, Sevgi
dc.contributor.authorIşık, Mesut
dc.contributor.authorAlp, Cemalettin
dc.contributor.authorDikici, Emrah
dc.contributor.authorKöksal, Ekrem
dc.contributor.authorKırboğa, Kevser Kübra
dc.contributor.authorRudrapal, Mithun
dc.contributor.authorRakshit, Gourav
dc.contributor.authorBeydemir, Şükrü
dc.contributor.authorKhan, Johra
dc.date.accessioned2025-07-17T12:24:57Z
dc.date.available2025-07-17T12:24:57Z
dc.date.issued2025
dc.departmentRektörlük
dc.description.abstractLaurus nobilis (LN), has traditional medicinal uses, and this study investigates its therapeutic potential by focusing on its phenolic content and bioactivities such as antioxidant, antidiabetic, and anticholinergic properties. Phenolic compounds play key roles in reducing oxidative stress and modulating enzymatic activities, relevant to metabolic and neurodegenerative disorders. Methods: LN leaf extracts were prepared via ethanol maceration, followed by filtration and concentration. Phenolic content was analyzed using LC-MS/MS. Antioxidant activity was assessed through ferric thiocyanate, DPPH, ABTS, and FRAP assays. Enzyme inhibition assays targeted AChE, BChE, and α-GLY, with IC50 values from dose-response curves. In silico analyses were conducted using molecular docking techniques to predict the binding mechanisms of identified phenolic compounds with the active sites of target enzymes, evaluating binding affinities and interaction profiles. Results: Vanillic acid and catechin hydrate were the most abundant phenolics. LN extract showed strong lipid peroxidation inhibition (50.53%) compared to Trolox (28.33%) and α-tocopherol (37.79%). Moderate radical scavenging and metal reduction potentials were observed. IC50 values were 2.57 µg/L for AChE, 3.78 µg/L for BChE, and 4.65 µg/L for α-GLY, indicating notable bioactivity. In silico studies confirmed strong binding affinities of phenolics to target enzymes. Discussion: LN extracts demonstrated promising antioxidant, antidiabetic, and anticholinergic activities, attributed to high phenolic content. Enzyme inhibition results suggest potential in managing metabolic and neurodegenerative disorders. In silico findings support these bioactivities, highlighting LN’s therapeutic potential.
dc.identifier.doi10.3389/fchem.2025.1541250
dc.identifier.issn22962646
dc.identifier.scopus86000075541
dc.identifier.urihttps://dx.doi.org/10.3389/fchem.2025.1541250
dc.identifier.urihttps://hdl.handle.net/20.500.12451/13354
dc.identifier.volume13
dc.identifier.wosWOS:001436879700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.institutionauthorDikici, Emrah
dc.language.isoen
dc.publisherFrontiers Media SA
dc.relation.ispartofFrontiers in Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntidiabetic
dc.subjectAntioxidant
dc.subjectLaurus NobilisL
dc.subjectMolecular Docking
dc.subjectMolecular Dynamics
dc.subjectPhenolic Content
dc.titleTherapeutic potential of Laurus nobilis extract by experimental and computational approaches: phenolic content and bioactivities for antioxidant, antidiabetic, and anticholinergic properties
dc.typeArticle

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