Effects of Morin on the Wnt, Notch1/Hes1, KI-67/3-Nitrotyrosine and Damage Signaling Pathways in Rats Subjected to Experimental Testicular Ischemia/Reperfusion

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dc.authorid0000-0001-7773-5978
dc.contributor.authorÖztürk, Ayşe Betül
dc.contributor.authorŞimşek, Hasan
dc.contributor.authorAkaras, Nurhan
dc.contributor.authorKandemir, Fatih Mehmet
dc.date.accessioned2025-07-11T10:43:54Z
dc.date.available2025-07-11T10:43:54Z
dc.date.issued2025
dc.departmentTıp Fakültesi
dc.description.abstractTesticular torsion, which occurs when the testicle rotates around the axis of the spermatic cord, is a serious cause of hospital admission, mostly in newborns and children, but also in adults. Oxidative stress is an important mediator of the development of complications. Morin has anti-inflammatory, anti-autophagic, and anti-apoptotic activities and especially strong antioxidant activity. This study aimed to determine the effects of Morin on testicular torsion injury. Methods: 35 Wistar rats were divided into 5 groups (n = 7): Control, Morin, I/R, I/R + MRN50, and I/R + MRN100. Parameters are effective in oxidative stress, inflammation, endoplasmic reticulum stress, apoptosis, and autophagy damage and Wnt pathway parameters, KI-67, and 3-NT levels were analyzed by biochemical, molecular, and histological methods. Results: I/R injury significantly increased oxidative stress (MDA, p < 0.001) and reduced antioxidant activity (GSH, SOD, CAT, GPx; p < 0.001). MRN administration reversed these effects, with higher doses showing greater improvement (p < 0.01 for CAT, p < 0.001 for others). Inflammation markers (NF-kB, IL-1β, TNF-α, COX-2, iNOS) were elevated in the I/R group, but MRN reduced their expression (p < 0.001). MRN also mitigated ER stress and reactivated the Wnt signaling pathway, particularly at 100 mg/kg (p < 0.001). Additionally, MRN reduced apoptosis (Caspase-3, Bax, p < 0.001) and autophagy (Beclin-1, LC3A, LC3B, p < 0.001), and improved testicular histology and sperm parameters. MRN treatment restored sperm density, motility, and viability (p < 0.05), with higher doses proving more effective. Conclusion: MRN has effects properties in testicular I/R injury by inhibiting many damage pathways and activating protective mechanisms.
dc.identifier.doi10.1007/s44411-025-00039-2
dc.identifier.endpage426
dc.identifier.issn00069248
dc.identifier.issue4
dc.identifier.scopus85217796052
dc.identifier.startpage407
dc.identifier.urihttps://dx.doi.org/10.1007/s44411-025-00039-2
dc.identifier.urihttps://hdl.handle.net/20.500.12451/13261
dc.identifier.volume126
dc.identifier.wosWOS:001465484100001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.institutionauthorÖztürk, Ayşe Betül
dc.institutionauthorŞimşek, Hasan
dc.institutionauthorAkaras, Nurhan
dc.institutionauthorKandemir, Fatih Mehmet
dc.institutionauthorid0000-0001-7773-5978
dc.language.isoen
dc.publisherSpringer International Publishing
dc.relation.ispartofBratislava Medical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectApoptosis
dc.subjectIschemia–reperfusion injury
dc.subjectMorin
dc.subjectTestis
dc.subjectWnt
dc.titleEffects of Morin on the Wnt, Notch1/Hes1, KI-67/3-Nitrotyrosine and Damage Signaling Pathways in Rats Subjected to Experimental Testicular Ischemia/Reperfusion
dc.typeArticle

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