Effects of Morin on the Wnt, Notch1/Hes1, KI-67/3-Nitrotyrosine and Damage Signaling Pathways in Rats Subjected to Experimental Testicular Ischemia/Reperfusion
dc.authorid | 0000-0001-7773-5978 | |
dc.contributor.author | Öztürk, Ayşe Betül | |
dc.contributor.author | Şimşek, Hasan | |
dc.contributor.author | Akaras, Nurhan | |
dc.contributor.author | Kandemir, Fatih Mehmet | |
dc.date.accessioned | 2025-07-11T10:43:54Z | |
dc.date.available | 2025-07-11T10:43:54Z | |
dc.date.issued | 2025 | |
dc.department | Tıp Fakültesi | |
dc.description.abstract | Testicular torsion, which occurs when the testicle rotates around the axis of the spermatic cord, is a serious cause of hospital admission, mostly in newborns and children, but also in adults. Oxidative stress is an important mediator of the development of complications. Morin has anti-inflammatory, anti-autophagic, and anti-apoptotic activities and especially strong antioxidant activity. This study aimed to determine the effects of Morin on testicular torsion injury. Methods: 35 Wistar rats were divided into 5 groups (n = 7): Control, Morin, I/R, I/R + MRN50, and I/R + MRN100. Parameters are effective in oxidative stress, inflammation, endoplasmic reticulum stress, apoptosis, and autophagy damage and Wnt pathway parameters, KI-67, and 3-NT levels were analyzed by biochemical, molecular, and histological methods. Results: I/R injury significantly increased oxidative stress (MDA, p < 0.001) and reduced antioxidant activity (GSH, SOD, CAT, GPx; p < 0.001). MRN administration reversed these effects, with higher doses showing greater improvement (p < 0.01 for CAT, p < 0.001 for others). Inflammation markers (NF-kB, IL-1β, TNF-α, COX-2, iNOS) were elevated in the I/R group, but MRN reduced their expression (p < 0.001). MRN also mitigated ER stress and reactivated the Wnt signaling pathway, particularly at 100 mg/kg (p < 0.001). Additionally, MRN reduced apoptosis (Caspase-3, Bax, p < 0.001) and autophagy (Beclin-1, LC3A, LC3B, p < 0.001), and improved testicular histology and sperm parameters. MRN treatment restored sperm density, motility, and viability (p < 0.05), with higher doses proving more effective. Conclusion: MRN has effects properties in testicular I/R injury by inhibiting many damage pathways and activating protective mechanisms. | |
dc.identifier.doi | 10.1007/s44411-025-00039-2 | |
dc.identifier.endpage | 426 | |
dc.identifier.issn | 00069248 | |
dc.identifier.issue | 4 | |
dc.identifier.scopus | 85217796052 | |
dc.identifier.startpage | 407 | |
dc.identifier.uri | https://dx.doi.org/10.1007/s44411-025-00039-2 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12451/13261 | |
dc.identifier.volume | 126 | |
dc.identifier.wos | WOS:001465484100001 | |
dc.identifier.wosquality | Q2 | |
dc.indekslendigikaynak | Scopus | |
dc.indekslendigikaynak | Web of Science | |
dc.institutionauthor | Öztürk, Ayşe Betül | |
dc.institutionauthor | Şimşek, Hasan | |
dc.institutionauthor | Akaras, Nurhan | |
dc.institutionauthor | Kandemir, Fatih Mehmet | |
dc.institutionauthorid | 0000-0001-7773-5978 | |
dc.language.iso | en | |
dc.publisher | Springer International Publishing | |
dc.relation.ispartof | Bratislava Medical Journal | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Apoptosis | |
dc.subject | Ischemia–reperfusion injury | |
dc.subject | Morin | |
dc.subject | Testis | |
dc.subject | Wnt | |
dc.title | Effects of Morin on the Wnt, Notch1/Hes1, KI-67/3-Nitrotyrosine and Damage Signaling Pathways in Rats Subjected to Experimental Testicular Ischemia/Reperfusion | |
dc.type | Article |