The Use of boron compounds for stabilization of lipase from pseudomonas aeruginosa ES3 for the detergent industry

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dc.contributor.authorSaraç, Nurdan
dc.contributor.authorUğur, Aysel
dc.contributor.authorBoran, Rukiye
dc.contributor.authorElgin, Emine Sonay
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:30:23Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:30:23Z
dc.date.issued2015
dc.departmentSağlık Hizmetleri Meslek Yüksekokulu
dc.description.abstractThis study aimed to characterize a lipase that is highly active and stable under typical washing conditions for use as a detergent ingredient by investigating the effects of various boron compounds on lipase stabilization under different conditions. In addition, the antimicrobial activity of the boron compounds used in enzyme stabilization was examined in order to obtain an effective antimicrobial detergent. A lipase-producing bacterium was isolated from kitchen wastewater samples using Rhodamine-B Agar medium and identified as Pseudomonas aeruginosa based on 16S rDNA sequence analysis. The ES3 lipase obtained from P. aeruginosa was purified, and the purified enzyme was found to have a molecular mass of 40 kDa. The enzyme showed optimal activity at pH 9.0-10.0 and 40 A degrees C and remained stable in the presence of various metal ions, surfactants and oxidizing agents. Moreover, the pH stability and thermostability of the enzyme was improved by the addition of boron compounds, which, when used as stabilizers in the incubation media, also increased the stability of the enzyme towards commercial detergents. Furthermore, the enzyme displayed properties comparable with the commercial product Lipolase(A (R)), which has shown excellent stability towards various commercial detergents. Finally, boron compounds used to stabilize the lipase were found to possess antimicrobial properties, suggesting that detergents incorporating these compounds will also exhibit antimicrobial activity when washing clothes and dishes.
dc.description.sponsorshipTUBITAK, The Scientific and Technical Research Council of Turkey [TBAG- 210T084]
dc.description.sponsorshipThis work was supported by TUBITAK, The Scientific and Technical Research Council of Turkey, through the Grant number TBAG- 210T084.
dc.identifier.doi10.1007/s11743-014-1653-7
dc.identifier.endpage285en_US
dc.identifier.issn1097-3958
dc.identifier.issn1558-9293
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage275en_US
dc.identifier.urihttps://doi.org/10.1007/s11743-014-1653-7
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6270
dc.identifier.volume18en_US
dc.identifier.wosWOS:000349915500010
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSPRINGER HEIDELBERG
dc.relation.ispartofJOURNAL OF SURFACTANTS AND DETERGENTS
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectPseudomonas Aeruginosa
dc.subjectLipase
dc.subjectBoron
dc.subjectStabilization
dc.subjectDetergent
dc.subjectAntimicrobial
dc.titleThe Use of boron compounds for stabilization of lipase from pseudomonas aeruginosa ES3 for the detergent industry
dc.typeArticle

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