Preparation of Zn2+-chelated poly(HEMA-MAH) cryogel for affinity purification of chicken egg lysozyme

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dc.contributor.authorDerazshamshir, Ali
dc.contributor.authorErgün, Bahar
dc.contributor.authorPeşint, Gözde
dc.contributor.authorOdabaşı, Mehmet
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:26:41Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:26:41Z
dc.date.issued2008
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractSupermacroporous poly(2-hydroxyethyl methacrylate) [poly(HEMA)]-based monolithic cryogel column was prepared by radical cryocopolymerization of HEMA with N-methacryloyl-L-histidine methyl ester (MAH) as functional comonomer and N,N'-methylene-bisacrylamide (MBAAm) as crosslinker directly in a plastic syringe for affinity purification of lysozyme from chicken egg white. The monolithic cryogel containing a continuous polymeric matrix having interconnected pores of 10-50 mu m size was loaded with Zn2+ ions to form the metal chelate with poly(HEMA-MAH) cryogel. Poly(HEMA-MAH) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly(HEMA-MAH) monolithic cryogel was 5.62 g H2O/g cryogel. Poly(HEMA-MAH) cryogel containing 45.8 mu mol MAH/g was used in the adsorption/desorption of lysozyme from aqueous solutions. The nonspecific adsorption of lysozyme was very low (7.5 mg/g). The maximum amount of lysozyme adsorption from aqueous solution in phosphate buffer was 209 mg/g at pH 7.0. It was observed that lysozyme could be repeatedly adsorbed and desorbed with the poly (HEMA-MAH) cyogel without significant loss of adsorption capacity. (C) 2008 Wiley Periodicals, Inc.
dc.identifier.doi10.1002/app.28345
dc.identifier.endpage2913en_US
dc.identifier.issn0021-8995
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage2905en_US
dc.identifier.urihttps://doi.org/10.1002/app.28345
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5679
dc.identifier.volume109en_US
dc.identifier.wosWOS:000257442900022
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley and Sons Limited
dc.relation.ispartofJournal of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAffinity Chromatography
dc.subjectProtein Purification
dc.subjectMetal Chelate
dc.subjectHistidine
dc.subjectCryogels
dc.titlePreparation of Zn2+-chelated poly(HEMA-MAH) cryogel for affinity purification of chicken egg lysozyme
dc.typeArticle

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