Hemostatic activities of nano/microporous bilayer dressings in a femoral artery bleeding rat model

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dc.authoridBayram, Cem -- 0000-0001-8717-4668;
dc.contributor.authorKarahaliloğu, Zeynep
dc.contributor.authorDemirbilek, Murat
dc.contributor.authorUlusoy, İbrahim
dc.contributor.authorGümüşkaya, Berrak
dc.contributor.authorDenkbaş, Emir Baki
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:29:22Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:29:22Z
dc.date.issued2016
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractRecently, an effective hemostatic dressing requirement has become a major problem in both the military and civilian world. Available hemostatic agents are too expensive, ineffective, unsafe, or complicated to use. Here, we evaluated the hemostatic efficacy of a nano/micro bilayer hemostatic dressing including a porous sublayer from chitosan (CTS) and bacterial cellulose (BC) and a nanofibrillar upper layer from silk fibroin (SF). In addition, several active agents rolled in coagulation cascade [vitamin K (Vit K), protamine sulfate (PS), kaolin (Kao), etc.] were doped to the sublayer of bilayer hemostatic agent and their activities were compared via in vivo and in vitro tests. Lactate dehydrogenase (LDH) activity test results demonstrated that BC/CTS, SF-coated BC/CTS, and Vit K K/BC/CTS, SF/phosphatidylcholine (PC)-coated BC/CTS showed higher LDH activity compared to standard gauze (p<0.005). In a femoral artery bleeding rat model, SF-coated PS/BC/CTS significantly reduced bleeding time (80 +/- 0.3 s) compared to standard gauze and kaolin-doped group (p<0.005). Blood loss and mortality rate with 2.3 g and 37.5% SF-coated BC/CTS and SF-coated PS/BC/CTS showed increased efficacy in achieving hemostasis compared to standard gauze. All the prepared hemostatic dressings except for kaolin-doped BC/CTS and standard gauze induced no inflammatory reaction in the tissue. All these data suggest that active agent-doped BC/CTS/SF-based bilayer hemostatic dressings have a great influence on the resulting hemostatic action. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43657.
dc.description.sponsorshipHacettepe University Teaching Staff Training Program Office [014BIYP604003]
dc.description.sponsorshipThis work was funded by the Hacettepe University Teaching Staff Training Program Office through a grant "014BIYP604003".
dc.identifier.doi10.1002/app.43657
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.issue28en_US
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/app.43657
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6160
dc.identifier.volume133en_US
dc.identifier.wosWOS:000374774100011
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiomaterials
dc.subjectBiomedical Applications
dc.subjectComposites
dc.subjectElectrospinning
dc.subjectFibers
dc.titleHemostatic activities of nano/microporous bilayer dressings in a femoral artery bleeding rat model
dc.typeArticle

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