Oxime-functionalized cryogel disks for catalase immobilization

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dc.authoridTuzmen, Nalan -- 0000-0001-9863-7023
dc.contributor.authorİnanan, Tülden
dc.contributor.authorTüzmen, Nalan
dc.contributor.authorKaripçin, Fatma
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:29:14Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:29:14Z
dc.date.issued2018
dc.departmentTeknik Bilimler Meslek Yüksekokulu
dc.description.abstractCatalase is a protective enzyme against oxidative stress and converts hydrogen peroxide into water and molecular oxygen. In the current study, catalase immobilization was applied onto the oxime-functionalized cryogel disks. Cryogel disks were produced by free radical polymerization. After cutting as circular disks, oxime ligand (4-biphenylchloroglyoxime, BPCGO) was attached and oxime-functionalized cryogel disks were obtained. After optimization of several immobilization parameters such as pH, initial catalase concentration, temperature and ionic strength, maximum catalase load was detected as 261.7 +/- 11.2 mg/g for cryogel disk at pH 5.0. Activity studies indicated that immobilization enhanced the enzyme activity in basic pH region, the temperature range of 15-35 degrees C and at ionic strengths between 0.2 and 1.0 M NaCI. Km was detected as 9.9 and 11.0 mM and V-max was 357.1 and 769.2 mu mol min(-1) for free and immobilized catalase, respectively. k(cat) and Km/k(cat) values showed that immobilization enhanced the catalytic efficiency. Storage stability experiments demonstrated that immobilization increased the usability period. Furthermore, catalase desorption was achieved by 1.0 M NaSCN at pH 8.0 successfully and catalase adsorption capacity of oxime-functionalized cryogel disk was decreased by 9.9% at the end of 5 adsorption-desorption cycle.
dc.identifier.doi10.1016/j.ijbiomac.2018.04.006
dc.identifier.endpage820en_US
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid29626600
dc.identifier.scopusqualityQ1
dc.identifier.startpage812en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2018.04.006
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6145
dc.identifier.volume114en_US
dc.identifier.wosWOS:000435056900092
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCatalase
dc.subjectImmobilization
dc.subjectOxime
dc.titleOxime-functionalized cryogel disks for catalase immobilization
dc.typeArticle

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