Unveiling genetic diversity among bacterial isolates suing SCoT markers

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dc.authorid0000-0002-4237-4858
dc.authorid0000-0002-3293-9894
dc.authorid0000-0002-3293-9894
dc.authorid0000-0003-2924-5434
dc.authorid0000-0002-7923-8892
dc.authorid0000-0001-8140-379X
dc.authorid0000-0002-4193-1990
dc.authorid0000-0002-2280-4582
dc.authorid0000-0001-6647-8136
dc.authorid0000-0001-5805-8000
dc.authorid0000-0002-3385-2520
dc.contributor.authorTekin, Fatih
dc.contributor.authorAltın, İrem
dc.contributor.authorAydın, Furkan
dc.contributor.authorAlkan, Mehtap
dc.contributor.authorOrel, Didem Canik
dc.contributor.authorArdıç, Mustafa
dc.contributor.authorGaši?, Katarina
dc.contributor.authorKovács, Tamás
dc.contributor.authorAllen, Caitilyn
dc.contributor.authorÖzer, Göksel
dc.date.accessioned2024-11-14T13:07:01Z
dc.date.available2024-11-14T13:07:01Z
dc.date.issued2024
dc.departmentMühendislik Fakültesi
dc.description.abstractThis study aimed to unveil the genetic diversity among 47 bacterial isolates from various species using start codon targeted (SCoT) markers. Six SCoT primers yielded 219 reproducible bands, with 89.04% exhibiting polymorphism. The amplification process generated 28 to 50 fragments per primer, with an average of 36.50. Genetic diversity was quantified using polymorphic information content (PIC) values ranging from 0.11 to 0.14, with SCoT32 showing the highest PIC (0.14) and SCoT23 the lowest (0.11). The resolving power (RP) index, used to assess primer discriminatory power, varied significantly, with SCoT23 demonstrating the highest RP (6.00) and SCoT29 the lowest (4.51). Comparative analysis with conventional markers like M13 and (GTG)5 revealed that certain SCoT primers exhibited superior PIC values, which indicates enhanced utility for interspecies differentiation. The high discrimination level achieved by SCoT primers underscores their effectiveness in genetic differentiation and biodiversity assessment within bacterial populations. This research highlights SCoT markers as powerful tools for microbial genetic studies, which offers valuable insights into bacterial diversity and provides a robust methodological framework for future investigations aimed at elucidating genetic variation and improving species identification. The application of SCoT markers represents a significant advancement in molecular techniques for bacterial characterization and phylogenetic analysis, demonstrating their potential to enhance our understanding of microbial genetics and evolution.
dc.identifier.doi10.1007/s00284-024-03938-9
dc.identifier.issn0343-8651
dc.identifier.issue12en_US
dc.identifier.scopusqualityQ3
dc.identifier.urihttps:/dx.doi.org/10.1007/s00284-024-03938-9
dc.identifier.urihttps://hdl.handle.net/20.500.12451/12649
dc.identifier.volume81en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofCurrent Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectBacteria
dc.subjectCodon
dc.subjectInitiator
dc.subjectDNA Primers
dc.subjectDNA
dc.subjectBacterial
dc.subjectGenetic Markers
dc.subjectGenetic Variation
dc.titleUnveiling genetic diversity among bacterial isolates suing SCoT markers
dc.typeArticle

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